The chemical environment at germination stage in Pinus halepensis somatic embryogenesis: implications in the morphological characteristics of the developed plantlets
DOI:
https://doi.org/10.24215/16699513e099Keywords:
acclimatation, somatic embryos, maltose, sacarose, conifersAbstract
Somatic embryogenesis is a promising method of propagation of conifers, but it requires optimized protocols according to the different stages of the process and the model species. Pinus halepensis Mill. (Aleppo pine) is a species widely used in reforestation and the somatic embryogenesis procedure was successfully developed, but even so, there is low germination and conversion of somatic embryos into plants. In this sense, promoting changes in the chemical environment in the germination stage is an alternative to increase germination rates and the consequent obtaining of somatic plants. Taking this into account, the objective of this work was to evaluate the influence of different carbohydrate sources applied during the germination stage of the somatic embryos of P. halepensis, on the success of this process and the morphology of the somatic plants obtained. A statistically significant increase in germination rates, total length of somatic plants, as well as principal root length was observed when somatic embryos were cultured in maltose-supplemented germination medium.
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