Influence of fetal bovine serum and amino acids on limb regeneration in the african clawed frog (xenopus laevis)

Authors

  • Giovanni Herrera-Sandoval Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango. Av. Universidad S/N, Fraccionamiento Filadelfia, Gómez Palacio, Durango, México. C.P. 35010
  • Fernando Alonzo-Rojo Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango. Av. Universidad S/N, Fraccionamiento Filadelfia, Gómez Palacio, Durango, México. C.P. 35010.
  • Jorge Sáenz-Mata Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango. Av. Universidad S/N, Fraccionamiento Filadelfia, Gómez Palacio, Durango, México. C.P. 35010.
  • Cristo Omar Puente-Valenzuela Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango. Av. Universidad S/N, Fraccionamiento Filadelfia, Gómez Palacio, Durango, México. C.P. 35010.
  • David Ramiro Aguillón-Gutiérrez Laboratorio de Bioindicadores, Centro de Investigación y Jardín Etnobiológico, Universidad Autónoma de Coahuila. Dr. Francisco González 37, Viesca, Coahuila, México. C.P. 27480.

Keywords:

Serum, amino acids, regeneration, limbs, Xenopus

Abstract

Biological regeneration is the capacity that certain organisms have to restore any lost or injured tissue. Regeneration has been studied in diverse biological models, for example the hydra (Hydra viridis), planarian (Planaria maculata, P. lugubris), and amphibians (Ambystoma mexicanum, Xenopus laevis). In this work, the influence of fetal bovine serum and amino acids in limb regeneration in African clawed frog (Xenopus laevis) was investigated. Forty specimens were studied on stage 66 which underwent an amputation in the proximal tibia-fibula area of the right hind limb, forming five groups (treatments) (amino acids, fetal bovine serum, application of physiological serum, mechanical stimulation and control) that were in experimental phase for a period of 78 days. Mortality and morphometric analysis were performed. The results showed no significant difference from the width and length of the spike under the influence of these treatments. Mortality was higher in the group where fetal bovine serum was applied and lower in the group receiving amino acids. It is believed that the regenerative capacity in the stage 66 is more influenced by the amputation site than the treatments.

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Published

2021-03-01

How to Cite

Herrera-Sandoval, G. ., Alonzo-Rojo, F. ., Sáenz-Mata, J., Puente-Valenzuela, C. O. ., & Aguillón-Gutiérrez, D. R. . (2021). Influence of fetal bovine serum and amino acids on limb regeneration in the african clawed frog (xenopus laevis). Revista Ciencias Morfológicas, 21(1), 1–10. Retrieved from https://revistas.unlp.edu.ar/Morfol/article/view/11394

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