Carious measurement and pulps modifications in rats undergoing habitual and cariogenic diets treated with collagenase type II

Authors

  • Alicia KOHLI Facultad de Odontología, Universidad Nacional de Rosario. Argentina.
  • Stella Maris PEZZOTTO
  • Leonor POLETTO
  • Hector DÁVILA

Abstract

Tooth can get ill by carious, with destruction of hard tissues exposed to germs, causing inflammation and pain, being the cariogenic diet a risk factor. To visualize the pulpodentinal components complex in rats, to compare measurement carious teeth methods and observe pulps modifications undergoing cariogenic diets. Rats inbred 'l' and 'e' lines were investigated, separated in G1 and G2 groups, nourished with a balanced rat-mouse diet. -G2 was supplemented with sucrose 10% in water ad libitum and then sacrificed sequentially. With magnifier, explorer and rhodamine cavitated and emerging carious were detected. Jaws were surgically separated, fixed in formalin 10%, demineralized in 8% nitric acid, treated with collagenase Type II, included, cut 5µ, colored and examined with microscope light 100-450 magnification. The basal area infiltration was considered absent when it was not visible, partial and total when they occupied 75% and 100% of pulp perimeter. Results were analyzed with Chi-square test and significance level 5%. Pulpodentinal complex remained joined, incipient carious proportion was higher and the basal was partial or fully infiltrated in line 'e' -G2, what could be due to environmental factors and cariogenic diet.

Keywords: carious, collagenase, cariogenic diet, dental pulp.

Downloads

Download data is not yet available.

How to Cite

KOHLI, A., PEZZOTTO, S. M., POLETTO, L., & DÁVILA, H. (2014). Carious measurement and pulps modifications in rats undergoing habitual and cariogenic diets treated with collagenase type II. Revista Ciencias Morfológicas, 16(1). Retrieved from https://revistas.unlp.edu.ar/Morfol/article/view/847

Issue

Section

Trabajos Originales