Análisis histoquímico de los glicoconjugados en las células mucosas de las branquias de Apareiodon affinis (STEINDACHNER, 1879) (CHARACIFORMES, PARADONTIDAE)
Resumen
Las características histoquímicas de las células mucosas de las branquias de Apareiodon affinis (Steindachner, 1879) (Characiformes, Paradontidae) fueron investigadas. Se utilizaron métodos para la localización y caracterización de glicoconjugados (GCs) con dioles vecinos oxidables, O-acil azúcares, ésteres orto-sulfatados y residuos de ácidos siálicos sin substitución o con substitución O-acilo en C7, C8 y/o C9. Se observaron células mucosas entre las células epiteliales del filamento y las laminillas secundarias. No se encontraron diferencias histoquímicas entre las células mucosas de las laminillas primarias y secundarias. Ellas poseen abundantes GCs con grupos carboxílicos y esteres O-sulfatados, junto con moderada proporción de GCs con dioles vecinos oxidables y baja cantidad de GCs con ácido siálico. Este trabajo demostró la heterogeneidad de los GCs de las células mucosas que podría asociarse con funciones diferentes como la lubricación, protección e inhibición de la invasión y proliferación de microorganismos patógenos y la regulación iónica.
Palabras clave: branquias, glicoconjugados, histoquímica, células mucosas, peces teleósteos.
Descargas
Los datos de descargas todavía no están disponibles.
Citas
Allen A. (1981). Structure and functions of gastrointestinal mucus. In: Johnson L.R. (Ed.), Physiology of the gastrointestinal tract.RavenPress,NewYork. P.617-639.
Arellano JM, Storch, V, Sarasquete C. (2004).Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. Journal ofAppliedIchthyology; 20:452-460.
Burkhardt-Holm P. (1997). Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin. Histochemical Journal; 29:893-899.
Culling CFA, Reid PE, Dunn WL. (1976). A new histochemical method for the identification and visualization of both side-chain acylated and non-acylated sialic acids. Journal of Histochemistry and Cytochemistry; 24:1225-1230.
Díaz AO,García AM,Devincenti CV,Goldemberg AL. (2001).Mucous cells inMicropogonias furnieri gills: histochemistry andultrastructure.AnatomiaHistologiaEmbryologia; 30:135-139.
Díaz AO, García AM, Escalante AH, Goldemberg AL. (2004). Glycoconjugates in the gills of Odontesthes bonariensis (Teleostei,Atherinopsidae).Biocell; 28(2):241.
Díaz AO,García AM,Devincenti CV,Goldemberg AL (2005a).Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri). Anatomia Histologia Embryologia; 34:117-122.
Díaz AO, García AM, Goldemberg AL. (2005b). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). ScientiaMarina; 69(4):545-553.
Díaz AO, García AM, Goldemberg AL. (2008). Glycoconjugates in the mucosa of the digestive tract of Cynoscion guatucupa:Ahistochemical study.ActaHistochemica;110: 76-85.
Domeneghini C, Pannelli Straini R, Veggetti A. (1998). Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adult ages. Histology and Histopathology; 13: 359-372.
Eiras-Stofella DR, Charvet-Almeida P, Fanta E, Casagrande Vianna AC. (2001). Surface ultrastructure of gills of themulletsMugil curema,M. liza andM. platanus (Mugilidae, Pisces). Journal ofMorphology; 247: 122-133.
Harrison JD, Auger DW, Paterson KL, Rowley PSA. (1987).Mucin histochemistry of submandibular and parotidsalivary glands ofman: light andelectronmicroscopy. TheHistochemical Journal; 19: 555-564.
Hossler FE, Harpole Jr. JH, King JA. (1986). The gill arch of the stripped bass, Morone saxatilis. I. Surface ultrastructure. Journal of SubmicroscopicalCytology; 18(3):519-528.
HughesGM. (1984).General anatomy of the gills. In:HoarWS,RandallDJ (ed.) Fish Physiology.Orlando: AcademicPress,pp1-72.
Jones R. (1977). Modification of mucus animal models of disease. Advances in Experimental Medicine andBiology; 89: 397-412.
Laurent P, Perry SF. (1991). Environmental effects on fish gillmorphology. Physiological Zoology; 64 (1):4-25.
Lev R, Spicer SS. (1964). Specific staining of sulphate groups with alcian blue at low pH. Journal of Histochemistry andCytochemistry; 12: 309.
Lillie RD, Fullmer HM. (1976). Chemical end groups. In: RD Lillie, Fullmer HM (eds) Histopathologic Technique andPracticalHistochemistry. NewYork:McGraw-Hill,pp. 217-326.
Machado MR, Fanta E. (2003). Effects of the organophosphorus methyl parathion on the branchial epithelium of a freshwater fishMetynnis roosevelti. Brazilian Archives of Biology and Technology; 46 (3): 361-372.
McManus JFA. (1948).Histological and histochemical uses of periodic acid. StainTechnology; 23: 99-108.
Miekle P, Richards GN, Yellowles D. (1988). Structural investigations on the mucus from six species of coral.Marine Biology; 99:187-193.
Mittal AK, Ueda T, Fujimori O, Yamada K. (1994). Histochemical analysis of glycoproteins in the unicellular glands in the epidermis of an Indian freshwater fish Mastacembelus pancalus Hamilton. Histochemical Journal; 26:666-677.
Mittal S, Pinky, Mittal AK. (2002). Characterisation of glycoproteins in the secretory cells in the operculum of an Indian hill stream fish Garra lamta (Hamilton) (Cyprinidae, Cypriniformes). Fish Physiology andBiochemistry; 33:35-48.
Mittal S, Pinky, Mittal AK. (2004). Operculum of peppered loach, Lepidocephalichthys guntea (Hamilton, 1922) (Cobitidae, Cypriniformes): a scanning electron microscopic and histochemical investigation. Belgian Journal ofZoology; 134(1):9-15.
Pavanelli CS. (2003). Family Parodontidae (Parodontids). In: Reis, RE, Kullander SO, Ferraris Jr CJ (eds) Check list the freshwater fishes of south andcentralAmerica.EDIPUCRS, PortoAlegre.pp46-50.
PearseAGE. (1985).Histochemistry.Theoretical andapplied.Vol. 2. Edinburgh:ChurchillLivingstone.
Pinky, Mittal S, Mittal AK. (2008). Glycoproteins in the epithelium of lips and associated structures of a hill stream fish Garra lamta (Cyprinidae, Cyprinoformes): a histochemical investigation. Anatomia HistologiaEmbryologia; 37: 101-113.
Reid PE, Culling CFA, Dunn WL. (1973). Saponification induced increase in the periodic acid Schiff reaction in the gastrointestinal tract. Mechanism and distribution of the reactive substance. Journal of Histochemistry andCytochemistry; 21: 473-483.
Sabóia-Moraes SMT,Hernández-Blázquez FJ,Mota DL, Bittencourt AM. (1996).Mucous cell types in the branchial epitheliumof the euryhaline fishPoecilia vivipara. Journal of FishBiology; 49:545-548.
Sarasquete C, González de Canales ML, Arellano JM, Muñoz-Cueto JA, Ribeiro L, Dinis MT. (1998). Histochemical study of skin and gills of Senegal sole, Solea senegalensis larvae and adults. Histology and Histopathology; 13:727-735.
Solanki TG, BenjaminM. (1982). Changes in the mucous cells of the gills, buccal cavity and epidermis of the nine-spinedstickleback,Pungitius pungitiusL., inducedby transferring the fish to seawater. Journal of FishBiology; 21:563-575.
Tibbets IR. (1997). The distribution and function of mucous cells and their secretions in the alimentary tract ofArrhamphus sclerolepis krefftii. Journalof FishBiology; 50:809-820.
Volz D, Reid PE, Park CM, Owen DA, Dunn WL. (1987). A new histochemical method for the selective periodate oxidation of total tissue sialic acids.Histochemical Journal; 19:311-318.
WhitearM,MittalAK. (1984). Surface secretions of the skin of Blennius (Lipophrys) pholis L. Journal of Fish Biology; 25:317-331.
Zaniboni-Filho E,Meurer S, ShibattaOA,NuñerAP deO. (2004).Catálogo ilustrado de peixes do alto rio Uruguai.Ed.daUFSC.TractebelEnergia. Florianópolis.
Arellano JM, Storch, V, Sarasquete C. (2004).Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. Journal ofAppliedIchthyology; 20:452-460.
Burkhardt-Holm P. (1997). Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin. Histochemical Journal; 29:893-899.
Culling CFA, Reid PE, Dunn WL. (1976). A new histochemical method for the identification and visualization of both side-chain acylated and non-acylated sialic acids. Journal of Histochemistry and Cytochemistry; 24:1225-1230.
Díaz AO,García AM,Devincenti CV,Goldemberg AL. (2001).Mucous cells inMicropogonias furnieri gills: histochemistry andultrastructure.AnatomiaHistologiaEmbryologia; 30:135-139.
Díaz AO, García AM, Escalante AH, Goldemberg AL. (2004). Glycoconjugates in the gills of Odontesthes bonariensis (Teleostei,Atherinopsidae).Biocell; 28(2):241.
Díaz AO,García AM,Devincenti CV,Goldemberg AL (2005a).Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri). Anatomia Histologia Embryologia; 34:117-122.
Díaz AO, García AM, Goldemberg AL. (2005b). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). ScientiaMarina; 69(4):545-553.
Díaz AO, García AM, Goldemberg AL. (2008). Glycoconjugates in the mucosa of the digestive tract of Cynoscion guatucupa:Ahistochemical study.ActaHistochemica;110: 76-85.
Domeneghini C, Pannelli Straini R, Veggetti A. (1998). Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adult ages. Histology and Histopathology; 13: 359-372.
Eiras-Stofella DR, Charvet-Almeida P, Fanta E, Casagrande Vianna AC. (2001). Surface ultrastructure of gills of themulletsMugil curema,M. liza andM. platanus (Mugilidae, Pisces). Journal ofMorphology; 247: 122-133.
Harrison JD, Auger DW, Paterson KL, Rowley PSA. (1987).Mucin histochemistry of submandibular and parotidsalivary glands ofman: light andelectronmicroscopy. TheHistochemical Journal; 19: 555-564.
Hossler FE, Harpole Jr. JH, King JA. (1986). The gill arch of the stripped bass, Morone saxatilis. I. Surface ultrastructure. Journal of SubmicroscopicalCytology; 18(3):519-528.
HughesGM. (1984).General anatomy of the gills. In:HoarWS,RandallDJ (ed.) Fish Physiology.Orlando: AcademicPress,pp1-72.
Jones R. (1977). Modification of mucus animal models of disease. Advances in Experimental Medicine andBiology; 89: 397-412.
Laurent P, Perry SF. (1991). Environmental effects on fish gillmorphology. Physiological Zoology; 64 (1):4-25.
Lev R, Spicer SS. (1964). Specific staining of sulphate groups with alcian blue at low pH. Journal of Histochemistry andCytochemistry; 12: 309.
Lillie RD, Fullmer HM. (1976). Chemical end groups. In: RD Lillie, Fullmer HM (eds) Histopathologic Technique andPracticalHistochemistry. NewYork:McGraw-Hill,pp. 217-326.
Machado MR, Fanta E. (2003). Effects of the organophosphorus methyl parathion on the branchial epithelium of a freshwater fishMetynnis roosevelti. Brazilian Archives of Biology and Technology; 46 (3): 361-372.
McManus JFA. (1948).Histological and histochemical uses of periodic acid. StainTechnology; 23: 99-108.
Miekle P, Richards GN, Yellowles D. (1988). Structural investigations on the mucus from six species of coral.Marine Biology; 99:187-193.
Mittal AK, Ueda T, Fujimori O, Yamada K. (1994). Histochemical analysis of glycoproteins in the unicellular glands in the epidermis of an Indian freshwater fish Mastacembelus pancalus Hamilton. Histochemical Journal; 26:666-677.
Mittal S, Pinky, Mittal AK. (2002). Characterisation of glycoproteins in the secretory cells in the operculum of an Indian hill stream fish Garra lamta (Hamilton) (Cyprinidae, Cypriniformes). Fish Physiology andBiochemistry; 33:35-48.
Mittal S, Pinky, Mittal AK. (2004). Operculum of peppered loach, Lepidocephalichthys guntea (Hamilton, 1922) (Cobitidae, Cypriniformes): a scanning electron microscopic and histochemical investigation. Belgian Journal ofZoology; 134(1):9-15.
Pavanelli CS. (2003). Family Parodontidae (Parodontids). In: Reis, RE, Kullander SO, Ferraris Jr CJ (eds) Check list the freshwater fishes of south andcentralAmerica.EDIPUCRS, PortoAlegre.pp46-50.
PearseAGE. (1985).Histochemistry.Theoretical andapplied.Vol. 2. Edinburgh:ChurchillLivingstone.
Pinky, Mittal S, Mittal AK. (2008). Glycoproteins in the epithelium of lips and associated structures of a hill stream fish Garra lamta (Cyprinidae, Cyprinoformes): a histochemical investigation. Anatomia HistologiaEmbryologia; 37: 101-113.
Reid PE, Culling CFA, Dunn WL. (1973). Saponification induced increase in the periodic acid Schiff reaction in the gastrointestinal tract. Mechanism and distribution of the reactive substance. Journal of Histochemistry andCytochemistry; 21: 473-483.
Sabóia-Moraes SMT,Hernández-Blázquez FJ,Mota DL, Bittencourt AM. (1996).Mucous cell types in the branchial epitheliumof the euryhaline fishPoecilia vivipara. Journal of FishBiology; 49:545-548.
Sarasquete C, González de Canales ML, Arellano JM, Muñoz-Cueto JA, Ribeiro L, Dinis MT. (1998). Histochemical study of skin and gills of Senegal sole, Solea senegalensis larvae and adults. Histology and Histopathology; 13:727-735.
Solanki TG, BenjaminM. (1982). Changes in the mucous cells of the gills, buccal cavity and epidermis of the nine-spinedstickleback,Pungitius pungitiusL., inducedby transferring the fish to seawater. Journal of FishBiology; 21:563-575.
Tibbets IR. (1997). The distribution and function of mucous cells and their secretions in the alimentary tract ofArrhamphus sclerolepis krefftii. Journalof FishBiology; 50:809-820.
Volz D, Reid PE, Park CM, Owen DA, Dunn WL. (1987). A new histochemical method for the selective periodate oxidation of total tissue sialic acids.Histochemical Journal; 19:311-318.
WhitearM,MittalAK. (1984). Surface secretions of the skin of Blennius (Lipophrys) pholis L. Journal of Fish Biology; 25:317-331.
Zaniboni-Filho E,Meurer S, ShibattaOA,NuñerAP deO. (2004).Catálogo ilustrado de peixes do alto rio Uruguai.Ed.daUFSC.TractebelEnergia. Florianópolis.
Descargas
Cómo citar
DIAZ, A. O., GARCIA, A. M., PINHEIRO Junior, C., BLAUTH DE LIMA, F., BRACCINI, M. del C., & GALARÇA GUIMARÃES, A. C. (2014). Análisis histoquímico de los glicoconjugados en las células mucosas de las branquias de Apareiodon affinis (STEINDACHNER, 1879) (CHARACIFORMES, PARADONTIDAE). Revista Ciencias Morfológicas, 10(1). Recuperado a partir de https://revistas.unlp.edu.ar/Morfol/article/view/936
Número
Sección
Trabajos Originales
Licencia
1. Política propuesta para revistas de acceso abierto
Los autores/as que publiquen en esta revista aceptan las siguientes condiciones:
- Los autores/as conservan los derechos de autor y ceden a la revista el derecho de la primera publicación, con el trabajo registrado con la licencia de atribución de Creative Commons, que permite a terceros utilizar lo publicado siempre que mencionen la autoría del trabajo y a la primera publicación en esta revista.
- Los autores/as pueden realizar otros acuerdos contractuales independientes y adicionales para la distribución no exclusiva de la versión del artículo publicado en esta revista (p. ej., incluirlo en un repositorio institucional o publicarlo en un libro) siempre que indiquen claramente que el trabajo se publicó por primera vez en esta revista.
- Se permite y recomienda a los autores/as a publicar su trabajo en Internet (por ejemplo en páginas institucionales o personales) despues del proceso de revisión y publicación, ya que puede conducir a intercambios productivos y a una mayor y más rápida difusión del trabajo publicado.
