Análisis histoquímico de los glicoconjugados en las células mucosas de las branquias de Apareiodon affinis (STEINDACHNER, 1879) (CHARACIFORMES, PARADONTIDAE)

Autores/as

  • Alcira Ofellia DIAZ
  • Alicia Mabel GARCIA
  • Clóvis PINHEIRO Junior
  • Fernanda BLAUTH DE LIMA
  • María del Carmen BRACCINI
  • Antonio Carlos GALARÇA GUIMARÃES

Resumen

Las características histoquímicas de las células mucosas de las branquias de Apareiodon affinis (Steindachner, 1879) (Characiformes, Paradontidae) fueron investigadas. Se utilizaron métodos para la localización y caracterización de glicoconjugados (GCs) con dioles vecinos oxidables, O-acil azúcares, ésteres orto-sulfatados y residuos de ácidos siálicos sin substitución o con substitución O-acilo en C7, C8 y/o C9. Se observaron células mucosas entre las células epiteliales del filamento y las laminillas secundarias. No se encontraron diferencias histoquímicas entre las células mucosas de las laminillas primarias y secundarias. Ellas poseen abundantes GCs con grupos carboxílicos y esteres O-sulfatados, junto con moderada proporción de GCs con dioles vecinos oxidables y baja cantidad de GCs con ácido siálico. Este trabajo demostró la heterogeneidad de los GCs de las células mucosas que podría asociarse con funciones diferentes como la lubricación, protección e inhibición de la invasión y proliferación de microorganismos patógenos y la regulación iónica.

Palabras clave: branquias, glicoconjugados, histoquímica, células mucosas, peces teleósteos.

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DIAZ, A. O., GARCIA, A. M., PINHEIRO Junior, C., BLAUTH DE LIMA, F., BRACCINI, M. del C., & GALARÇA GUIMARÃES, A. C. (2014). Análisis histoquímico de los glicoconjugados en las células mucosas de las branquias de Apareiodon affinis (STEINDACHNER, 1879) (CHARACIFORMES, PARADONTIDAE). Revista Ciencias Morfológicas, 10(1). Recuperado a partir de https://revistas.unlp.edu.ar/Morfol/article/view/936

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