Histochemical analysis of glycoconjugates in the branchial mucous cells of Apareiodon affinis (STEINDACHNER, 1879) (CHARACIFORMES, PARADONTIDAE)
Abstract
The histochemical characteristics of the mucous cells located in the gills of the fish Apareiodon affinis (Steindachner, 1879) (Characiformes, Paradontidae) were investigated. Several methods for the localization and characterization of glycoconjugates (GCs) with oxidizable vicinal diols, O -acyl sugars, O- sulphate esters and sialic acids residues without O-acyl substitution or with O-acyl substitution at C7, C8 or C9 were employed. Mucous cells were observed among the epithelial cells of the filament and the gill lamellae. No histochemical differences were detected between the mucous cells of primary and secondary lamellae. They contained high amounts of GCs with carboxyl groups and O-sulphate esters, together with moderate amounts of GCs with oxidizable vicinal diols, and low amounts of GCs with sialic acids. This work demonstrated the heterogeneity of the mucous cells GCs, which could be associated with different functions such as lubrication, protection and inhibition of the invasion and proliferation of pathogenic microorganisms and ionic regulation.
Key words: gills, glycoconjugates, histochemistry, mucous cells, teleost fish.
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References
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Arellano JM, Storch, V, Sarasquete C. (2004).Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. Journal ofAppliedIchthyology; 20:452-460.
Burkhardt-Holm P. (1997). Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin. Histochemical Journal; 29:893-899.
Culling CFA, Reid PE, Dunn WL. (1976). A new histochemical method for the identification and visualization of both side-chain acylated and non-acylated sialic acids. Journal of Histochemistry and Cytochemistry; 24:1225-1230.
Díaz AO,García AM,Devincenti CV,Goldemberg AL. (2001).Mucous cells inMicropogonias furnieri gills: histochemistry andultrastructure.AnatomiaHistologiaEmbryologia; 30:135-139.
Díaz AO, García AM, Escalante AH, Goldemberg AL. (2004). Glycoconjugates in the gills of Odontesthes bonariensis (Teleostei,Atherinopsidae).Biocell; 28(2):241.
Díaz AO,García AM,Devincenti CV,Goldemberg AL (2005a).Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri). Anatomia Histologia Embryologia; 34:117-122.
Díaz AO, García AM, Goldemberg AL. (2005b). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). ScientiaMarina; 69(4):545-553.
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Domeneghini C, Pannelli Straini R, Veggetti A. (1998). Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adult ages. Histology and Histopathology; 13: 359-372.
Eiras-Stofella DR, Charvet-Almeida P, Fanta E, Casagrande Vianna AC. (2001). Surface ultrastructure of gills of themulletsMugil curema,M. liza andM. platanus (Mugilidae, Pisces). Journal ofMorphology; 247: 122-133.
Harrison JD, Auger DW, Paterson KL, Rowley PSA. (1987).Mucin histochemistry of submandibular and parotidsalivary glands ofman: light andelectronmicroscopy. TheHistochemical Journal; 19: 555-564.
Hossler FE, Harpole Jr. JH, King JA. (1986). The gill arch of the stripped bass, Morone saxatilis. I. Surface ultrastructure. Journal of SubmicroscopicalCytology; 18(3):519-528.
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Lev R, Spicer SS. (1964). Specific staining of sulphate groups with alcian blue at low pH. Journal of Histochemistry andCytochemistry; 12: 309.
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Mittal AK, Ueda T, Fujimori O, Yamada K. (1994). Histochemical analysis of glycoproteins in the unicellular glands in the epidermis of an Indian freshwater fish Mastacembelus pancalus Hamilton. Histochemical Journal; 26:666-677.
Mittal S, Pinky, Mittal AK. (2002). Characterisation of glycoproteins in the secretory cells in the operculum of an Indian hill stream fish Garra lamta (Hamilton) (Cyprinidae, Cypriniformes). Fish Physiology andBiochemistry; 33:35-48.
Mittal S, Pinky, Mittal AK. (2004). Operculum of peppered loach, Lepidocephalichthys guntea (Hamilton, 1922) (Cobitidae, Cypriniformes): a scanning electron microscopic and histochemical investigation. Belgian Journal ofZoology; 134(1):9-15.
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PearseAGE. (1985).Histochemistry.Theoretical andapplied.Vol. 2. Edinburgh:ChurchillLivingstone.
Pinky, Mittal S, Mittal AK. (2008). Glycoproteins in the epithelium of lips and associated structures of a hill stream fish Garra lamta (Cyprinidae, Cyprinoformes): a histochemical investigation. Anatomia HistologiaEmbryologia; 37: 101-113.
Reid PE, Culling CFA, Dunn WL. (1973). Saponification induced increase in the periodic acid Schiff reaction in the gastrointestinal tract. Mechanism and distribution of the reactive substance. Journal of Histochemistry andCytochemistry; 21: 473-483.
Sabóia-Moraes SMT,Hernández-Blázquez FJ,Mota DL, Bittencourt AM. (1996).Mucous cell types in the branchial epitheliumof the euryhaline fishPoecilia vivipara. Journal of FishBiology; 49:545-548.
Sarasquete C, González de Canales ML, Arellano JM, Muñoz-Cueto JA, Ribeiro L, Dinis MT. (1998). Histochemical study of skin and gills of Senegal sole, Solea senegalensis larvae and adults. Histology and Histopathology; 13:727-735.
Solanki TG, BenjaminM. (1982). Changes in the mucous cells of the gills, buccal cavity and epidermis of the nine-spinedstickleback,Pungitius pungitiusL., inducedby transferring the fish to seawater. Journal of FishBiology; 21:563-575.
Tibbets IR. (1997). The distribution and function of mucous cells and their secretions in the alimentary tract ofArrhamphus sclerolepis krefftii. Journalof FishBiology; 50:809-820.
Volz D, Reid PE, Park CM, Owen DA, Dunn WL. (1987). A new histochemical method for the selective periodate oxidation of total tissue sialic acids.Histochemical Journal; 19:311-318.
WhitearM,MittalAK. (1984). Surface secretions of the skin of Blennius (Lipophrys) pholis L. Journal of Fish Biology; 25:317-331.
Zaniboni-Filho E,Meurer S, ShibattaOA,NuñerAP deO. (2004).Catálogo ilustrado de peixes do alto rio Uruguai.Ed.daUFSC.TractebelEnergia. Florianópolis.
Arellano JM, Storch, V, Sarasquete C. (2004).Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. Journal ofAppliedIchthyology; 20:452-460.
Burkhardt-Holm P. (1997). Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin. Histochemical Journal; 29:893-899.
Culling CFA, Reid PE, Dunn WL. (1976). A new histochemical method for the identification and visualization of both side-chain acylated and non-acylated sialic acids. Journal of Histochemistry and Cytochemistry; 24:1225-1230.
Díaz AO,García AM,Devincenti CV,Goldemberg AL. (2001).Mucous cells inMicropogonias furnieri gills: histochemistry andultrastructure.AnatomiaHistologiaEmbryologia; 30:135-139.
Díaz AO, García AM, Escalante AH, Goldemberg AL. (2004). Glycoconjugates in the gills of Odontesthes bonariensis (Teleostei,Atherinopsidae).Biocell; 28(2):241.
Díaz AO,García AM,Devincenti CV,Goldemberg AL (2005a).Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri). Anatomia Histologia Embryologia; 34:117-122.
Díaz AO, García AM, Goldemberg AL. (2005b). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). ScientiaMarina; 69(4):545-553.
Díaz AO, García AM, Goldemberg AL. (2008). Glycoconjugates in the mucosa of the digestive tract of Cynoscion guatucupa:Ahistochemical study.ActaHistochemica;110: 76-85.
Domeneghini C, Pannelli Straini R, Veggetti A. (1998). Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adult ages. Histology and Histopathology; 13: 359-372.
Eiras-Stofella DR, Charvet-Almeida P, Fanta E, Casagrande Vianna AC. (2001). Surface ultrastructure of gills of themulletsMugil curema,M. liza andM. platanus (Mugilidae, Pisces). Journal ofMorphology; 247: 122-133.
Harrison JD, Auger DW, Paterson KL, Rowley PSA. (1987).Mucin histochemistry of submandibular and parotidsalivary glands ofman: light andelectronmicroscopy. TheHistochemical Journal; 19: 555-564.
Hossler FE, Harpole Jr. JH, King JA. (1986). The gill arch of the stripped bass, Morone saxatilis. I. Surface ultrastructure. Journal of SubmicroscopicalCytology; 18(3):519-528.
HughesGM. (1984).General anatomy of the gills. In:HoarWS,RandallDJ (ed.) Fish Physiology.Orlando: AcademicPress,pp1-72.
Jones R. (1977). Modification of mucus animal models of disease. Advances in Experimental Medicine andBiology; 89: 397-412.
Laurent P, Perry SF. (1991). Environmental effects on fish gillmorphology. Physiological Zoology; 64 (1):4-25.
Lev R, Spicer SS. (1964). Specific staining of sulphate groups with alcian blue at low pH. Journal of Histochemistry andCytochemistry; 12: 309.
Lillie RD, Fullmer HM. (1976). Chemical end groups. In: RD Lillie, Fullmer HM (eds) Histopathologic Technique andPracticalHistochemistry. NewYork:McGraw-Hill,pp. 217-326.
Machado MR, Fanta E. (2003). Effects of the organophosphorus methyl parathion on the branchial epithelium of a freshwater fishMetynnis roosevelti. Brazilian Archives of Biology and Technology; 46 (3): 361-372.
McManus JFA. (1948).Histological and histochemical uses of periodic acid. StainTechnology; 23: 99-108.
Miekle P, Richards GN, Yellowles D. (1988). Structural investigations on the mucus from six species of coral.Marine Biology; 99:187-193.
Mittal AK, Ueda T, Fujimori O, Yamada K. (1994). Histochemical analysis of glycoproteins in the unicellular glands in the epidermis of an Indian freshwater fish Mastacembelus pancalus Hamilton. Histochemical Journal; 26:666-677.
Mittal S, Pinky, Mittal AK. (2002). Characterisation of glycoproteins in the secretory cells in the operculum of an Indian hill stream fish Garra lamta (Hamilton) (Cyprinidae, Cypriniformes). Fish Physiology andBiochemistry; 33:35-48.
Mittal S, Pinky, Mittal AK. (2004). Operculum of peppered loach, Lepidocephalichthys guntea (Hamilton, 1922) (Cobitidae, Cypriniformes): a scanning electron microscopic and histochemical investigation. Belgian Journal ofZoology; 134(1):9-15.
Pavanelli CS. (2003). Family Parodontidae (Parodontids). In: Reis, RE, Kullander SO, Ferraris Jr CJ (eds) Check list the freshwater fishes of south andcentralAmerica.EDIPUCRS, PortoAlegre.pp46-50.
PearseAGE. (1985).Histochemistry.Theoretical andapplied.Vol. 2. Edinburgh:ChurchillLivingstone.
Pinky, Mittal S, Mittal AK. (2008). Glycoproteins in the epithelium of lips and associated structures of a hill stream fish Garra lamta (Cyprinidae, Cyprinoformes): a histochemical investigation. Anatomia HistologiaEmbryologia; 37: 101-113.
Reid PE, Culling CFA, Dunn WL. (1973). Saponification induced increase in the periodic acid Schiff reaction in the gastrointestinal tract. Mechanism and distribution of the reactive substance. Journal of Histochemistry andCytochemistry; 21: 473-483.
Sabóia-Moraes SMT,Hernández-Blázquez FJ,Mota DL, Bittencourt AM. (1996).Mucous cell types in the branchial epitheliumof the euryhaline fishPoecilia vivipara. Journal of FishBiology; 49:545-548.
Sarasquete C, González de Canales ML, Arellano JM, Muñoz-Cueto JA, Ribeiro L, Dinis MT. (1998). Histochemical study of skin and gills of Senegal sole, Solea senegalensis larvae and adults. Histology and Histopathology; 13:727-735.
Solanki TG, BenjaminM. (1982). Changes in the mucous cells of the gills, buccal cavity and epidermis of the nine-spinedstickleback,Pungitius pungitiusL., inducedby transferring the fish to seawater. Journal of FishBiology; 21:563-575.
Tibbets IR. (1997). The distribution and function of mucous cells and their secretions in the alimentary tract ofArrhamphus sclerolepis krefftii. Journalof FishBiology; 50:809-820.
Volz D, Reid PE, Park CM, Owen DA, Dunn WL. (1987). A new histochemical method for the selective periodate oxidation of total tissue sialic acids.Histochemical Journal; 19:311-318.
WhitearM,MittalAK. (1984). Surface secretions of the skin of Blennius (Lipophrys) pholis L. Journal of Fish Biology; 25:317-331.
Zaniboni-Filho E,Meurer S, ShibattaOA,NuñerAP deO. (2004).Catálogo ilustrado de peixes do alto rio Uruguai.Ed.daUFSC.TractebelEnergia. Florianópolis.
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DIAZ, A. O., GARCIA, A. M., PINHEIRO Junior, C., BLAUTH DE LIMA, F., BRACCINI, M. del C., & GALARÇA GUIMARÃES, A. C. (2014). Histochemical analysis of glycoconjugates in the branchial mucous cells of Apareiodon affinis (STEINDACHNER, 1879) (CHARACIFORMES, PARADONTIDAE). Revista Ciencias Morfológicas, 10(1). Retrieved from https://revistas.unlp.edu.ar/Morfol/article/view/936
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