Ultramicroscopic changes observed in sperm cats (felis catus) frozen-thawed

Authors

  • Maria Candela BONAURA Cátedra de Reproducción Animal, Laboratorio de Teriogenología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC).
  • Susana JURADO Servicio Central de Microscopía Electrónica, Faculta de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • Romina NUÑEZ FAVRE Cátedra de Reproducción Animal, Laboratorio de Teriogenología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata. CONICET
  • Romina PRADERIO Cátedra de Reproducción Animal, Laboratorio de Teriogenología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • Claudia TITTARELLI Cátedra de Reproducción Animal, Laboratorio de Teriogenología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • María Alejandra STORNELLI

Abstract

Cryopreservation procedure causes sperm structural disorders decreasing fertility. The aim was to study ultramicroscopic damage observed in frozen-thawed sperm. Mixed breed tomcats (n=16) that underwent bilateral orchiectomy at a municipal public pet shelter were used in the study. Sperm samples were obtained by cutting the cauda epididymis and frozen using Tris base extender with dimetilformamide 0.5% and 4.5% of glycerol or without dimetilformamide and 5% glycerol. Sperm samples were observed in a transmission electron microscope JEM 1200 EX II. For each treatment 100 heads and 100 tails were evaluated. The percentage of intact sperm was higher in fresh spermatozoa than frozen-thawed sperm (80.75±5.64vs10.25±1.24; 91.75±2.13vs59.27±3.92; p<0.05).Frozen-thawed procedure produce ultrastructural damage.

Keywords: cat, sperm, cryopreservation, ultramicroscopy.

Downloads

Download data is not yet available.

How to Cite

BONAURA, M. C., JURADO, S., NUÑEZ FAVRE, R., PRADERIO, R., TITTARELLI, C., & STORNELLI, M. A. (2014). Ultramicroscopic changes observed in sperm cats (felis catus) frozen-thawed. Revista Ciencias Morfológicas, 15(2). Retrieved from https://revistas.unlp.edu.ar/Morfol/article/view/820

Issue

Section

Trabajos Originales

Most read articles by the same author(s)