Optimizacion de una técnica para la detección de patologías virales en Pleoticus Muelleri (Bate, 1988) en el estuario de Bahía Blanca, Argentina
Keywords:
virus, Pleoticus muelleri, WSSV, TSV, YHVAbstract
Trade in aquatic animals calls for the implementation of controls for the detection of pathogenic viruses in line with the regulations of the OIE, the World Organization for Animal Heath. In relation specifically to trade in crustaceans, the OIE identifies the following diseases: White Spot syndrome virus disease (WSSV); Yellow Head Virus disease (YHV); and Taura syndrome virus disease (TSV), all of which are highly dangerous. The purpose of this study was to determine whether these viruses occur in wild populations of Pleoticus muelleri in Bahía Blanca estuary, Argentina, using biochemical, molecular biology and genetics methods. The results show that the methods used for DNA and RNA extraction and purification from the tissue and hemolymph of these crustaceans, and the techniques for their capture, packaging and transportation to the laboratory have been optimized. Tests were properly validated by negative and positive controls. None of the named diseases were found in the wild populations of Pleoticus muelleri. The methodology followed in the present research not only enables early detection and diagnosis of the three viral pathologies but also ensures an infection-free status with the concomitant health benefits for the region.
Downloads
References
Bayot ,B, Sonnenholzner S., Ochoa , X, J Guerrerro , J, Vera, T, Calderón , J, De Blas I, CornejoGrunauer , Mp. Stern , S Y Ollevier .F., 2008. An online operational alert system for the early detection of shrimp epidemics at the regional level based on real-time production. Aquaculture 277: 164-173.
Boada, M, Donato M.D. y Rodulfo H., 2008. Detection of the Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in Asymtomatic Cultured White Shrimp, Litopenaeus vannamei (Boone), in Venezuela. Rev. Científica, FCV-LUZ . XVIII, Nº 1: 7 – 11.
Bonami, J.R.; Hasson, K.W.; Mari, J.; Poulos, B.T. and D.V. Lightner.,1997. Taura Syndrome of marine penaeid shrimp: characterization of the viral agent. J. Gen. Virol. 78: 313-319.
Boschi E.E., 1997. Las pesquerías de crustáceos decápodos en el litoral de la República Argentina. Invest. Mar. 25: 19-40.
Chapman, R. W., Browdy, Cl, Savin, S, Prior, S Y Wenner. E., 2004. Sampling and evaluation of white spot syndrome virus in commercially important Atlantic penaeid shrimp stocks diseases of aquatic organisms. Dis Aquat Org, 59: 179–185.
Fenucci, J.L., Mallo, J.C. Y Galarza, C.M., 2002. Larval culture and growth in ponds of the Argentine shrimp Pleoticus muelleri Bate (Crustacea, Penaeoidea). Aquaculture World Aquaculture Society. San Diego. California. USA, pág. 106.
Harán, N, Mallo Jc., Fenucci .J., 2004. Density influence on growth and development of the petasma in juvenile shrimps Pleoticus muelleri (Decapoda, Penaeoidea). Invest. Mar. 32(1): 11-18.
Hasson K.W., Lightner D.V., Mohney L.L., Redman R.M., Poulos B.T., Mari J. Y Bonami J.R., 2009. The geographic distribution of Taura Syndrome Virus (TSV) in the Americas: determination by histology and in situ hybridization using TSV-specific DNA probes. Aquaculture, 171:13–26.
Lightner D.V., 1999. A Handbook of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultured Penaeid Shrimp. En: Lightner D.V. (Ed.). World Aquaculture Society, Baton Rouge, Louisiana, USA. 304 p.
Lightner, D.V., 1996. Epizootiology, distribution and the impact on international trade of two penaeid shrimp viruses in the Americas. Revue Scientifique et Technique Office International des Epizooties 15: 579-601.
Lotz J.M., Anton, L.S. y Soto M.A., 2005. Effect of chronic Taura syndrome virus infection on salinity tolerance of Litopenaeus vannamei. Dis. Aquat. Org., 65, 75–78.
Mallo, J.C. y Fenucci J.L., 2004. Alimentación de protozoeas de Pleoticcus muelleri Bate utilizando diferentes microencapsulados y especies de microalgas Rev. de Biol Mar y Oceanografia 39 (1): 13-19.
Martínez, B.; Koloffon Tella, S.; Mcgladdery, S. Y Enríquez, R., 2008. Políticas, marco de trabajo y lineamientos del Comité Interamericano de Sanidad de los Animales Acuáticos Rev. Sci. Tech. Off. int. Epiz., 27 (1):65-70..
Martorelli, S. R., Overstreet, R. M. and Jovonovich, J. A., 2010. First report of viral pathogens WSSV and IHHNV in argentine crustaceans. Bulletin Of Marine Science, 86(1): 117–131
Martorelli S.R., 2002. Parásitos y epibiontes del Langostino Pleoticus muelleri (Bate 1888) en el Atlántico Sud-occidental. Congreso Virtual de Acuicultura: 647-665. http://www.revistaaquatic.com/civa2002/ CIVA.
Mcclennen C., 2004. White spot syndrome virus. The economic, environmental and technical implications on the development of Latin American shrimp farming. Master of Arts in Law and Diplomacy Thesis, 106 pp.
OIE, 2008. Código Sanitario para los animales acuáticos. http://www.oie.int/esp/normesfcode/E_INDEX. SAGPyA Pesca Marítima, 2008. Especies. http://www.sagpya.mecon.gov.ar/
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2010 Ana M. Roccamo, Patricia M. Cervellini, M. Cintia Piccolo, Francisco J. Barrantes
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Starting in 2022 (Vol. 43 number 2) articles will be published in the journal under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license (CC BY-NC-SA 4.0)
According to these terms, the material can be shared (copied and redistributed in any medium or format) and adapted (remixed, transformed and created from the material another work), provided that a) the authorship and the original source of its publication (journal and URL of the work), b) is not used for commercial purposes and c) the same license terms are maintained.
Prior to this date the articles were published in the journal under a Creative Commons Attribution license (CC BY)
In both cases, the acceptance of the originals by the journal implies the non-exclusive assignment of the economic rights of the authors in favor of the editor, who allows reuse, after editing (postprint), under the license that corresponds according to the edition.
Such assignment means, on the one hand, that after its publication (postprint) in the GEOACTA Magazine of the Association of Geophysicists and Geodesists, the authors can publish their work in any language, medium and format (in such cases, it is requested that it be recorded that the material was originally published in this journal); on the other, the authorization of the authors for the work to be harvested by SEDICI, the institutional repository of the National University of La Plata, and to be disseminated in the databases that the editorial team considers appropriate to increase visibility. of the publication and its authors.
Likewise, the journal encourages the authors so that after their publication in the Journal of the Association of Geophysicists and Geodesists, they deposit their productions in other institutional and thematic repositories, under the principle that offering society scientific production and Unrestricted academic scholarship contributes to a greater exchange of global knowledge.
