Conidial production of Purpureocillium lilacinum LPSC # 876 on solid substrates. Effect on Nacobbus aberrans in tomato plants

Authors

  • María C. Gortari Universidad Nacional de La Plata, Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Argentina
  • Roque A. Hours Universidad Nacional de La Plata, Argentina

Keywords:

biological control, nematophagous fungi, root-knot nematodes, solid-state fermentation, agricultural residues

Abstract

Purpureocillium lilacinum (Thom) Samson (Luangsa-ard et al.) has been reported for the control of the root-knot disease caused by Nacobbus aberrans. Artisanal production of biocontrol fungal agents is carried out on solid substrates. The objectives of the present work were to analyze: 1) the production of conidia by P. lilacinum LPSC # 876 grown on different solid substrates with various C/N ratios, and 2) the effect of some of the fermented products obtained containing P. lilacinum conidia on tomato plants (Lycopersicon esculentum Mill.) cv. Platense (cultivated in pots under greenhouse conditions) inoculated with N. aberrans. Conidial production varied depending on the substrates utilized (rice grains, bran and husk, oyster mushroom residue, shrimp shell and sawdust); rice bran (alone or in combination with other substrates) yielded the best results. The maximum conidia production (1010 conidia/g of fermented product) corresponded to C/N ratios from 16:1 to 29:1. Significantndifferences were found on the effect of P. lilacinum onto N. aberrans. Tomato plants inoculated with conidia produced on rice bran mixed with oyster mushroom residue showed the lowest amount of galls, egg masses and eggs per egg masse. Nevertheless, an antagonistic effect on the nematode was not evidenced clearly. Rice bran contains the necessary nutrients for a good conidial production by P. lilacinus although its combination with oyster mushroom residue resulted in higher yields. It is necessary to redesign the experimental strategies to demonstrate the antagonism of P. lilacinus onto N. aberrans.

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Published

2016-12-15

How to Cite

Gortari, M. C., & Hours, R. A. (2016). Conidial production of Purpureocillium lilacinum LPSC # 876 on solid substrates. Effect on Nacobbus aberrans in tomato plants. Journal of the Agronomy College, 115(2), 239-249. https://revistas.unlp.edu.ar/revagro/article/view/19942