Insect marking for biological studies
DOI:
https://doi.org/10.24215/16699513e093Keywords:
radioisotopes, stable isotopes, trace elements, dispersion, behaviorAbstract
The marking technique allows studying the behavior and ecological interactions of insects, such as patterns of dispersion and movement, territoriality, handling and consumption of food, vector-parasite associations and food chains and webs. This review describes the advantages and disadvantages of markers, highlighting three methods whose differential is that they remain in the insect regardless of the stage of life. Radioisotopes minimize direct manipulation and trauma to insects, can be applied to large populations and are easily traceable, but their disposal limits field application. Stable isotopes that occur naturally in the environment are not radioactive and are related to ecological studies of trophic levels and metabolic processes, among the most used elements, carbon isotopes mainly reflect the diet of animals; nitrogen isotopes reflect agricultural practices (extensive x intensive) and in part diet and oxygen and hydrogen isotopes are linked to the isotopic composition of water, which, in turn, is dependent on geographic factors such as altitude, climate and latitude . Trace elements are also used as internal (non-radioactive) markers and can vary according to geographic location, changing the amounts found in plants and insects. Therefore, the search for a better methodology to detect the correlation of insects with man and the environment depends on the type of study to be carried out.
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