Penetración de fosfomicina en explantes intestinales
Keywords:
Fosfomicina, Deoxinivalenol, Explantes intestinales, CerdosAbstract
Fosfomycin (FOS) is an antibiotic used in pig farms for treatment and prevention of infections caused by resistant bacteria during post-weaning period. Antibiotics and non-toxic concentrations of such mycotoxins as deoxynivalenol (DON) are frequently found in the diet of animals. These compounds can establish interactions in the intestinal tract which affect and/or modify the penetration of FOS to enterocytes. The aim of this study was to culture intestinal explants from jejuna of pigs and to apply this model to the study of intracellular penetration of FOS in presence or absence of DON. The use of intestinal explants reduces the number of animals sacrificed per experiment and preserves normal intestinal histological structure in vitro. The results showed that there was no statistically significant difference in the intracellular concentration of FOS between explants incubated with 580 ppm FOS and explants incubated with 580 ppm FOS and 1 ppm DON. The Cmax was 12 ppm and the tmax was 2 h. Our study proved that only the 2% of the antibiotic is intracellularly accumulated and that the intracellular concentration of FOS is not affected by the presence of non-toxic concentrations of DON.
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