STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG
Keywords:
canine, spermatozoo, semen, hemocytometry, spectrophotometryAbstract
The aims of this study were to determine the repeatability of semen measures carried out by spectrophotometry and to compare them with that of the hemocytometer, to design a standard spectrophotometric curve for canine seminal concentration, and to correlate results obtained by both methods. For these purposes, 9 ejaculates were obtained from 5 normal male dogs. Each ejaculate was divided into four parts, and then diluted 1:25, 1:50, 1:75 in distilled water; the last fraction was not diluted. All the samples were measured three consecutive times by each method. Repeatability and correlation coefficient were calculated for both methods. The coefficient of variation of the spectrophotometer and hemocytometer was 7.8% and 16.9%, respectively and the correlation between absorbance and sperm count was r=0.89 (p < 0.01). These findings further present spectrophotometry not only as a rapid and low cost method but also precise for semen evaluation in this species.
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