El método experimental en Antropología Biológica. Historia y actualidad en Argentina / The experimental method in Biological Anthropology. History and current status in Argentina
Resumen
La práctica experimental en Antropología no difiere en esencia de la de otras ciencias. Consiste en reproducir, bajo condiciones controladas, un hecho natural previamente observado, con el objetivo de estudiar su causa y efecto. Cuando se considera al hombre inmerso en un ambiente bio-físico-sociocultural, se torna dificultoso establecer patrones de diferenciación debido a la multiplicidad de factores actuantes y sus probables interacciones. En este contexto, surgió la necesidad de incorporar la metodología experimental en los estudios antropológicos. En el presente trabajo se realiza una revisión histórica y actual del empleo del método experimental en Antropología Biológica. Se destacan los trabajos pioneros de Sherwood Washburn y Samuel Detwiler, realizados en anfibios, y los de Melvin Moss, en ratas, para estudiar el crecimiento craneano, entre otros. En Argentina, en particular, se reconoce a Héctor Pucciarelli como el responsable de incorporar dicha metodología en los estudios bioantropológicos en la década de los años 70 y de consolidar un grupo de trabajo y distintas líneas de investigación que continúan su desarrollo en la actualidad.
Palabras clave: antropología biológica; método experimental; Argentina
Experimental methods in Anthropology are not different from those of other sciences. They are used to simulate previously observed natural events under controlled conditions, in order to study their causes and effects. Given that humans are immersed in a biological, physical and socio-cultural environment, establishing differentiation patterns proves more difficult because of the multiplicity of factors involved and their probable interactions. In this context, it became necessary to incorporate the experimental method in anthropological studies. In our study we review the past and present of the experimental method in Biological Anthropology, namely, the pioneering work of Sherwood Washburn and Samuel Detwiler, in amphibians, and that of Melvin Moss, in rats, to study cranial growth. In Argentina, Héctor Pucciarelli is recognized as the first to introduce this methodology in bioanthropological studies in the seventies. He also consolidated a work group and developed different lines of research still in progress today.
Keywords: biological anthropology; experimental method; Argentina
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Cesani MF. 2004. Influencia de la subnutrición proteicocalórica transgeneracional sobre el crecimiento de la descendencia en la rata (Rattus norvegicus albinus var. Wistar). Un estudio de antropología biológica experimental. Tesis Doctoral Inédita. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata.
Cesani MF, Luna ME, Castro LE, Quintero FA, Fucini MC, Villanueva M, Prio MV, Guimarey LM, Oyhenart EE. 2010. Retardo prenatal del crecimiento óseo y crecimiento compensatorio durante la lactancia. Un estudio experimental. En: Gutierrez-Redomero E, Sanches Andrés A, Galera Olmo V, editores. Diversidad Humana y Antropología Aplicada. Alcalá de Henares: SEAF, Universidad de Alcalá y IUICP. p. 169-176.
Cesani MF, Orden AB, Oyhenart EE, Zucchi M, Muñe MC, Pucciarelli HM. 2006. Growth of functional cranial components in rats submitted to intergenerational undernutrition. J Anat 209: 137-147. doi:10.1111/j.1469-7580.2006.00603.x
Cesani MF, Orden AB, Zucchi M, Oyhenart EE, Muñe MC, Pucciarelli HM. 2001. Influencia de la alimentación durante la lactancia sobre la desnutrición intergeneracional. Un estudio experimental. Rev Arg Antrop Biol 3:101-111.
Cesani MF, Orden B, Zucchi M, Muñe MC, Oyhenart EE, Pucciarelli HM. 2003. Effect of undernutrition on the cranial growth of the rat. An intergenerational study. Cells Tissues Organs 174:129-135. doi:10.1159/000071153
Cesani MF, Oyhenart EE, Pucciarelli HM. 2014. Effect of intergenerational chronic undernutrition on ponderal, and linear growth. ISRN Nutr. doi: 10.1155/2014/453460
Churchill SE, Schmitt D. 2003. Biomechanics in paleoanthropology: engineering and experimental approaches to the investigation of behavioral evolution in the genus Homo. En: Harcourt C, Crompton R, editores. New perspectives in Primate evolution and behavior. London: Linnaean Society. p. 59-90.
Cónsole GM, Jurado SB, Oyhenart EE, Ferese C, Pucciarelli HM, Gómez Dumm CL. 2001a. Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys. Braz J Med Biol Res 34:65-74. doi 10.1590/s0100-879x2001000100008
Cónsole GM, Oyhenart EE, Jurado SB, Riccillo FL, Pucciarelli HM, Gómez Dumm CL. 2001b. Effect of undernutrition on cranial components and somatotrophlactotroph pituitary populations in the squirrel monkey (Saimiri sciureus boliviensis). Cells Tissues Organs 168:272-284. doi:10.1159/000047844
Dahinten SL. 1988. Influencia de la malnutrición proteínicocalórica en ratas, sobre la diferenciación del fenotipo craneano. Tesis Doctoral Inédita. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata.
Dahinten SL, Pucciarelli HM. 1981. Effect of age, sex and nutrition on discontinuous traits of rat skull. Acta Anat 110:159-163. doi:10.1159/000145425
Dahinten SL, Pucciarelli HM. 1983. Effects of protein calorie malnutrition during suckling and post-weaning periods on discontinuous cranial traits in rats. Am J Phys Anthropol 60:425-430. doi:10.1002/ajpa.1330600403
Dahinten SL, Pucciarelli HM. 1986. Variations in sexual dimorphism in the skulls of rats subjected to malnutrition, castration, and treatment with gonadal hormones. Am J Phys Anthropol 71:63-67. doi:10.1002/ajpa.1330710108
Dahinten SL, Pucciarelli HM, Moreno FR. 1988. Effect of gonadal activity on cranial dimorphism of the rat. Acta Anat 132:324-326. doi:10.1159/000146596
Dressino V. 1991. Desarrollo craneofacial en Saimiri sciureus (Cebidae) y su alteración por factores nutricionales. Tesis Doctoral Inédita. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata.
Dressino V, Pucciarelli HM. 1996. Efecto nutricional sobre el crecimiento craniofacial de Saimiri sciureus (Cebidae). Un estudio experimental. Rev Arg Antrop Biol 1:98-112.
Dressino V, Pucciarelli HM. 1997. Cranial growth in Saimiri sciureus (Cebidae) and its alteration by nutritional factors: a longitudinal study. Am J Phys Anthropol 102:545-554. doi:10.1002/(sici)1096-8644(199704)102:4<545::aid-ajpa8>3.0.co;2-q
Dressino V, Pucciarelli HM. 1999. Growth of functional cranial components in Saimiri sciureus boliviensis (Cebidae). A longitudinal study. Growth Dev Aging 63:111-127.
Dressino V, Orden B, Oyhenart EE. 2002. Sexual responses to intrauterine stress: body and brain growth. Clin Exp Obstet Gynecol 29:100-102.
Elftman H, Manter J. 1935. Chimpanzee and human feet in bipedal walking. Am J Phys Anthropol 20:69-79. doi:10.1002/ajpa.1330200109
Fleagle JG. 1979. Primate positional behavior and anatomy: naturalistic and experimental approaches. En: Morbeck ME, Preuschoft H, Gomberg N, editores. Environment, behavior, and morphology: dynamic interactions in Primates. New York: Gustav Fischer. p. 313-326.
Garn SM. 1962. The newer physical anthropology. Am Anthropol 64:917-918. doi:10.1525/aa.1962.64.5.02a00010
Garn SM. 1963. Physical anthropology today. Am J Phys Anthropol 21:225-226. doi:10.1002/ajpa.1330210214
Gonzalez PN, Hallgrímsson B, Oyhenart E. 2011a. Developmental plasticity in the covariance structure of the skull: effects of prenatal stress. J Anat 218:243-257. doi:10.1111/j.1469-7580.2010.01326.x
Gonzalez PN, Oyhenart EE, Hallgrímsson B. 2011b. Effects of environmental perturbations during postnatal development on the phenotypic integration of the skull. J Exp Zool Mol Dev Evol 314: 547-561. doi:10.1002/jez.b.21430
Guimarey LM, Oyhenart EE, Quintero F, Fucini MC. 2003. Body weight recovery in intrauterine growth-retarded rats treated with growth hormone. Clin Exp Obstet Gynecol 30:51-56.
Guimarey LM, Quintero FA, Orden AB, Fucini MC, Oyhenart EE. 2005. Catch up in body weight of IUGR rats castrated and treated with growth hormone. Growth Dev Aging 69:89-99.
Hildebrand GY. 1931. Studies in the masticatory movements of the lower jaw. Scand Arch Physiol Suppl 61:1-190.
Hylander WL. 1977. In vivo bone strain in the mandible of Galago crassicaudatus. Am J Phys Anthropol 46:309-326. doi: 10.1002/ajpa.1330460212
Hylander WL. 1979. An experimental analysis of temporomandibular joint reaction force in macaques. Am J Phys Anthropol 51:433-456. doi:10.1002/ajpa.1330510317
Lemelin P, Schmitt D. 2007. Origins of grasping and locomotor adaptations in primates: comparative and experimental approaches using an opossum model. En: Ravosa MJ, Dagosto M, editores. Primate origins: adaptations and evolution. New York: Kluwer Academic. p. 329-380. doi:10.1007/978-0-387-33507-0_10
Linneo C. 1735. Sistema naturae. Leiden: Theodorum Haak. Little MA, Sussman RW. 2010. History of biological anthropology. En: Larsen CS, editor. A companion to biological anthropology. Oxford: Wiley-Blackwell. p. 13-38. doi:10.1002/9781444320039.ch1
Luna ME. 2012. Influencia de los factores nutricionales sobre el crecimiento pre y post natal: Un estudio experimental. Tesis Doctoral Inédita. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata.
Luna ME, Quintero FA, Cesani MF, Fucini MC; Prío V, Guimarey LM, Oyhenart EE. 2014. Craniofacial catchup growth in intrauterine growth retarded rats following postnatal nutritional rehabilitation. Clin Exp Obstet Gynecol 41:530-533.
Mielke JH, Konigsberg LW, Relethford JH. 2006. Human biological variation. New York: Oxford University Press. doi:10.1086/661144
Moss ML. 1954. Growth of the calvaria in the rat. The determination of osseous morphology. Am J Anat 94:333-362. doi:10.1002/aja.1000940302
Moss ML. 1955. Morphological changes in the growing rat skull following the administration of cortisone acetate. Proc Soc Exp Biol 89:648-650. doi:10.3181/00379727-89-21905
Moss ML. 1958. Rotations of cranial components in the growing rat and their experimental alteration. Acta Anat 32:65-86. doi: 10.1159/000141314
Moss ML. 1985. The application of the finite element method to the analysis of craniofacial growth and form. Acta Morphol Neerl Scand 23:37-56.
Moss ML, Baer MJ. 1956. Differential growth of the rat skull. Growth 20:107-120.
Moss ML, Pucciarelli HM, Moss-Salentijn L, Skalak R, Bose A, Goodall C, Sen K, Morgan B, Winick M. 1987b. Effects of pre-weaning undernutrition on 21 day-old male rat skull form as described by the finite element method. Gegenbaurs Morphol Jahrb 133:837-868.
Moss ML, Skalak R, Patel H, Sen K, Moss-Salentijn L, Shinozuka M, Vilmann H. 1985. Finite element method modeling of craniofacial growth. Am J Orth 89: 453-472. doi:10.1016/0002-9416(85)90084-3
Moss ML, Vilmann H. 1978. Studies on orthocephalization of the rat head. I. A model system for the study of adjustive cranial growth processes. Gegenbaurs Morphol Jahrb 124:559-579.
Moss ML, Vilmann H, Moss-Salentijn L, Sen K, Pucciarelli HM, Skalak R. 1987a. Studies on orthocephalization: growth behavior of the rat skull in the period 13-49 days as described by the finite element method. Am J Phys Anthropol 72:323-342. doi:10.1002/ajpa.1330720306
Moss ML, Young RW. 1960. A functional approach to craniology. Am J Phys Anthropol 18: 281–292. doi: 10.1002/ajpa.1330180406
Orden AB. 1997. Ontogenia del dimorfismo sexual y su alteración por factores nutricionales. Un estudio de antropología biológica experimental. Tesis Doctoral Inédita. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata.
Orden AB, Pucciarelli HM, Cesani MF, Muñe MC, Oyhenart EE, Zucchi M, Villanueva M, Rodríguez RR, Pons ER. 2003. Variación craneofacial en ratas sometidas a subnutrición transgeneracional moderada. En: Aluja MP, Malgosa A, Nogués RM, editores. Antropología y Biodiversidad. Barcelona: Bellaterra, p.423-429.
Orden AB, Pucciarelli HM, Muñe MC, Guimarey LM, Villanueva ME, Rodríguez RR, Pons ER. 1998. Efecto de las hormonas gonadales sobre el dimorfismo sexual en substrato de desnutrición. Acta Physiol Pharmacol Ther Latinoam 48:157-163.
Oyhenart EE. 1988. Estudio experimental del dimorfismo craneano racial y sexual y su alteración por efecto del mestizaje. Tesis Doctoral Inédita. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata.
Oyhenart EE, Pucciarelli HM. 1991. The influence of gonadic hormones on skull differences in rats malnourished during lactation. Acta Physiol Pharmacol Ther Latinoam 41:287-293.
Oyhenart EE, Pucciarelli HM. 1992. Sexual cranial dimorphism in malnourished rats treated with growth hormone. Growth Dev Aging 56:179-184.
Oyhenart EE, Cesani Rossi MF, Pucciarelli HM. 1999. Influencia del retardo del crecimiento intrauterino sobre la diferenciación craneana postnatal. Rev Arg Antrop Biol 2:135-150.
Oyhenart EE, Guimarey LM, Fucini MC, Quintero FA, Orden B. 2002. Effects of bilateral uterine ligation on skeletal growth in rats. Clin Exp Obstet Gynecol 29:121-125.
Oyhenart EE, Guimarey LM, Quintero FA, Fucini MC, Orden AB. 2003a. Efecto de la hormona de crecimiento sobre el retardo prenatal del crecimiento. En: Aluja MP, Malgosa A, Nogués RM, editores. Antropología y biodiversidad. Barcelona: Bellaterra, p. 455-461.
Oyhenart EE, Muñe MC, Pucciarelli HM. 1996. Influencia de la malnutrición intrauterina tardía sobre el crecimiento corporal y el desarrollo craneofacial al nacimiento. Rev Arg Antrop Biol 1:113-116.
Oyhenart EE, Muñe MC, Pucciarelli HM. 1998. Influence of intrauterine blood supply on cranial growth and sexual dimorphism at birth. Growth Dev Aging 52:115-122.
Oyhenart EE, Orden B, Fucini MC, Muñe MC, Pucciarelli HM. 2003b. Sexual dimorphism and postnatal growth of intrauterine growth retarded rats. Growth Dev Aging 67:73-83.
Oyhenart EE, Quintero FA, Orden AB, Fucini MC, Guimarey LM, Carino M, Ferese C, Cónsole GM. 2008. Catch-up Growth in intrauterine growth retarded rats: its correlation with histomorphometric changes of the pituitary somatotrope cells. Eur J Anat 12:115-122.
Oyhenart EE, Sobrero MS, Pucciarelli HM. 1994. Heredity, nutrition and craniofacial differentiation in weanling rats. Am J Hum Biol 6:277-282. doi:10.1002/ajhb.1310060302
Pucciarelli HM. 1973. Experimental technique for cranial deformation in growing rats. Acta Physiol Latinoamer 23:141-147.
Pucciarelli HM. 1974a. El método experimental en antropología biológica. Etnía 19:1-7.
Pucciarelli HM. 1974b. The influence of experimental deformation on neurocranial wormian bones in rats. Am J Phys Anthropol 41:29-38. doi:10.1002/ajpa.1330410105
Pucciarelli HM. 1980. The effects of race, sex and nutrition on craneofacial differentiation in rats. A multivariate analysis. Am J Phys Anthropol 53:359-368. doi:10.1002/ajpa.1330530307
Pucciarelli HM. 1981. Growth of the functional components of the rat skull and its alteration by nutritional effects. A multivariate analysis. Am J Phys Anthropol 56:33-41. doi:10.1002/ajpa.1330560104
Pucciarelli HM. 1989. Contribución al concepto de Antropología Biológica. Rev Antropología 7:27-31.
Pucciarelli HM. 1991. Nutrición y morfogénesis craneofacial. Una contribución de la Antropología Biológica Experimental. Interciencia 16: 248-253.
Pucciarelli HM, Cesani MF, Orden AB, Oyhenart EE, Zucchi M, Muñe MC. 2006. Intergenerational effects on food intake of the rats. Int J Anthrop 21:167-177.
Pucciarelli HM, Dressino V, Niveiro MH. 1990. Changes in skull components of the squirrel monkey evoked by growth and nutrition: An experimental study. Am J Phys Anthropol 81:535-543. doi:10.1002/ajpa.1330810409
Pucciarelli HM, Goya RG. 1983. Effects of postweaning malnutrition on the weight of the head components in rats. Acta Anat 115:231-237. doi:10.1159/000145694
Pucciarelli HM, Moss ML, Skalak R, Moss-Salentijn L, Sen K, Vilmann H. 1987. Aplicación del Método de los Elementos Finitos en estudios de diferenciación craneofacial. Revista del Museo de La Plata. Sección Antropología 9:1-20.
Pucciarelli HM, Muñe MC, Oyhenart EE, Orden AB, Villanueva ME, Rodríguez RR, Pons ER. 2000. Growth of skeletal components in the young squirrel monkey (Saimiri sciureus boliviensis). A longitudinal experiment. Am J Phys Anthropol 112:57-68. doi:10.1002/(sici)1096-8644(200005)112:1<57::aidajpa7>3.0.co;2-q
Pucciarelli HM, Orden AB, Cesani MF, Oyhenart EE, Muñe MC, Zucchi M. 2001. Relative food intake of rats submitted to a moderate transgenerational undernutrition. Growth DevAging 65:83-93.
Pucciarelli HM, Oyhenart EE. 1987a. Effects of maternal food restriction during lactation on craniofacial growth in weanling rats. Am J Phys Anthropol 72: 67-75. doi:10.1002/ajpa.1330720109
Pucciarelli HM, Oyhenart EE. 1987b. Influence of food restriction during gestation on craniofacial growth in weanling rats. Acta Anat 129:182-187. doi:10.1159/000146397
Pucciarelli HM, Oyhenart EE, Muñe MC. 1983. Alterations in protein and mineral contents of rat skull bones, evoked by different protein levels of the diet. Acta Anat 117:331-338. doi:10.1159/000145805
Pucciarelli HM, Oyhenart EE, Terreros MC. 1984. Variations of rat skull bone robusticity evoked by malnutrition. Am J Phys Anthropol 64:119-124. doi:10.1002/ajpa.1330640204
Quintero FA. 2008. Efecto del retardo prenatal de crecimiento sobre el crecimiento posnatal de ratas. Modificaciones inducidas por hormonas de crecimiento y sexuales. Tesis Doctoral Inédita. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata.
Quintero FA, Castro LE, Luna ME, Guimarey LM, Cesani MF, Fucini MC, Villanueva M, Prio V, Oyhenart EE. 2012. Growth of functional cranial components in rats with intrauterine growth retardation after treatment with growth hormone. Eur J Orthod 34:710-718. doi:10.1093/ejo/cjr101
Quintero FA, Orden AB, Fucini MC, Oyhenart EE, Guimarey LM. 2005. Bone growth in IUGR rats treated with growth hormone: a multivariate approach. Eur J Anat 9:149-154.
Quintero F, Orden B, Guimarey L, Fucini M, Oyhenart E. 2004. Skeletal maturation in intrauterine growth retarded rats treated with growth hormone. Clin Exp Obstet Gynecol 31:130-132.
Resnick O, Morgane PJ. 1984. Generational effect of protein malnutrition in the rat. Dev Brain Res 317:219-227. doi:10.1016/0165-3806(84)90099-3
Riesenfeld A. 1966. The effect of experimental bipedalism and upright posture in the rat and their significance for the study of human evolution. Acta Anat 65:449-521. doi:10.1159/000142902
Riesenfeld A. 1970. The effect of environmental factors on tooth development: an experimental investigation. Acta Anat 77:188-215. doi:10.1159/000143541
Riesenfeld A. 1973. The effect of extreme temperatures and starvation on the body proportions of the rat. Am J Phys Anthropol 39:427-59. doi:10.1002/ajpa.1330390311
Scarano ER, Pucciarelli HM, Crivos M, Prati M. 1994. Estado actual de la experimentación antropológica en Argentina. Interciencia 19:191-195.
Schmitt D, Wall CE, Lemelin P. 2008. Experimental comparative anatomy in physical anthropology: the contributions of Dr. William L. Hylander to studies of skull form and function. En: Vinyard C, Ravosa MJ, Wall C, editores. Primate craniofacial function and biology. Durham: Springer. p 3-16. doi:10.1007/978-0-387-76585-3_1
Stini WA. 2010. Sherwood Washburn and the ‘‘new physical anthropology’’. En: Little MA, Kennedy KAR, editores. Histories of american physical anthropology in the twentieth century. New York: Rowman and Littlefield. p. 173-186. doi:10.1086/656834
van der Klaauw CJ. 1952. Size and position of the functional components of the skull. A Contribution to the knowledge of the architecture of the skull, based on data in the literature. Arch Neerl Zool 9:1-556. doi:10.1163/187530152X00017
Vilmann H, Moss ML. 1979. Spatial position of the lateral semicircular canal in 14 60-day-old rat heads. Scand J Dent Res 87:171-177. doi:10.1111/j.1600-0722.1979.tb00669.x
Vilmann H, Moss ML. 1981. Studies on orthocephalization VII. Behavior of the rat cranial frame in the period between 1 day before birth to 14 days after birth. Acta Anat 109: 157-160. doi:10.1159/000145378
Vuguin PM. 2002. Animals models for assessing the consequences of intrauterine growth retardation on subsequent glucose metabolism of the offspring: a review. J Matern Fetal Neonatal Med 11:254-257. doi:10.1080/jmf.11.4.254.257
Vugin PM. 2007. Animal models for small for gestational age and fetal programing of adult disease. Horm Res 68:113-123. doi:10.1159/000100545
Washburn SL. 1946a. The effect of facial paralysis on the growth of the skull of rat and rabbit. Anat Rec 94:163-168. doi:10.1002/ar.1090940205
Washburn SL. 1946b. The effect of removal of the zygomatic arch in the rat. J Mammal 27:169-172. doi:10.2307/1375234
Washburn SL. 1947. The relation of the temporal muscle to the form of the skull. Anat Rec 99:239-248. doi: 10.1002/ar.1090990303
Washburn SL. 1951. The new physical anthropology. Trans NY Acad Sci 13:298-304. doi:10.1111/j.2164-0947.1951.tb01033.x
Washburn SL, Detwiler SB. 1943. An experiment bearing on the problems of physical anthropology. Am J Phys Anthropol 1:171-190. doi:10.1002/ajpa.1330010211
Wigglesworth JS. 1964. Experimental growth retardation in the foetal rat. J Pathol 88:1-13. doi:10.1002/path.1700880102
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