Lipid content and Chlorella homosphaerabiomass grown under heterotrophic conditionsand different carbon and nitrogen concentrations
Keywords:
Chlorella homosphaera, microalga oil, glucose, nitrogen, fed batch cultureAbstract
The increasing interest in the study of microalgae cultivation has been oriented to biomass production in order to use it in food preparation, as well as in bioactive compounds with high medicinal value in the world markets. They are especially used in functional food development, owing to their nutritional and pharmaceutical properties. The aim of this study was to evaluate the effect of different concentrations of the carbon source (glucose) and nitrogen source (N-NO3) in heterotrophic growth and lipid productivity of microalga Chlorella homosphaera. The fractional factorial design used was of type 32, which offers 9 combinations, only 5 were used and one of them in triplicate.
The factors were glucose (5, 10 and 15 g.L-1) and N-NO3 (82.5; 165 and 247.5 mg.L-1) concentrations and the response variables were the total lipid and the biomass concentrations. The treatment with 5 g.L-1 of glucose and 247.5 mg.L-1 of N-NO3 showed the highest biomass yield (1.22 g.L-1) and the highest lipid productivity (13.07 mg.L-1.d-1), predominantly composed of palmitic (C16:0 - 23.6 %w/w) and linoleic (C18:1n9 - 22.4 %w/w) fatty acids. The interaction between the concentration of glucose and N-NO3 concentration influenced significantly the responses of total lipids and the maximum concentration of biomass. The microalga studied produced a higher concentration of lipids when grown with the lowest concentration of glucose associated with a higher concentration of nitrogen experimental design, which is very promising at the industrial.
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