Morphological characterization of seeds and seedlings of Lotus tenuis and Lotus corniculatus in order to differentiate species in seed samples

Authors

  • Mónica Liliana Murcia Universidad Nacional de Mar del Plata, Argentina
  • Anna Peretti Universidad Nacional de Mar del Plata, Argentina
  • Silvina San Martino Universidad Nacional de Mar del Plata, Argentina

Keywords:

seeds, seedlings, identification, morphology

Abstract

The aim was to morphologically characterize the seeds and the seedlings of Lotus tenuis and Lotus corniculatus in order to differentiate samples of seeds of both species. Twenty eight samples were studied to observe: in seeds, the morphology of the hilar region; in seedlings, the central leaflet pubescence of the first leaf and the epicotyl pubescence, the morphology of the epidermal cells, and the length of the occlusive and epidermal cells in leaflets and cotyledons. The chromosome counting in root meristematic cells allowed to verify species, 2n=2x=12 in Lt and 2n=4x = 24 in Lc. Data were analyzed by homogeneity of proportions and compare means. The hilar region of the seeds presented three shapes with variable frequencies, therefore it was not valid to differentiate species. The pubescence was null or very scarce in Lt and clearly visible in Lc, being this a valid feature to differentiate the species. The shape of the inferior epidermis cells in leaflets and cotyledons was not useful to differentiate the species. The size of the epidermal cells on the upper and the lower surface and the size of guard cells in the upper epidermis of cotyledon did not provide diagnostic value to differentiate both species. Therefore, the useful characteristics to differentiate and establish proportions between the two species on samples of seeds, were the chromosome count and the pubescence of epicotyl and central leaflet of the first leaf.

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References

Arambarri, A. & M. Colares. 1993. Lotus corniculatus and L. tenius Waldst et Kit (leguminosae) anatomy of leaf. Lotus newsletter 24. pp 38-40.

Burkart, A. 1952. Las leguminosas argentinas silvestres y cultivadas. 2a ed.ACME, Buenos Aires. 659 pp.

Celotto, A.I. & A.M. Sanso. 2008. Análisis cromosómico aplicado a la diferenciación de Lotus tenuis y L. corniculatus (Fabaceae). Análisis de semillas 3(7). pp 93-96.

D'Ambrogio de Argüeso, A. 1986. Manual de Técnicas en Histología Vegetal. Bs.As. Ed. Hemisferio Sur S.A. 83 pp.

Dawson, C.D.R. 1941. Tetrasomic inheritance in Lotus corniculatus L. Journal of Genetics, 42. pp 49-72.

Dizeo de Strittmatter, C., M. Kade & A. Valverde. 1985 Compared morphology of the aril region in Lotus corniculatus L. and Lotus tenuis Waldst. et Kit. seeds. Lotus Newsletter 16:8.

Galussi, A.A., P.D. Reinoso, L.R. Zimmerman, G.I. Soldá & L.M Lui. 2006. Identificación de cultivares de Lotus spp. por análisis de proteínas seminales. Revista de la Facultad de Agronomía, La Plata 106 (1). pp 21-26.

International Seed Testing Association (ISTA). 2008. International Rules for Seed Testing. Bassersdorf. Switzerland. 441 pp.

Kade, M., M.L. Wagner, C.D. Strittmatter, R.A. Ricco & A.A. Gurni. 1997. Identification of Lotus tenuis and L. corniculatus by flavonols. Seed Science and Technology 25. pp 585-587.

Lagler, J.C. 2003. Lotus. Un género que no acaba en dos especies. Revista Forrajes y granos 62. pp 72-76.

Maddaloni, J. & L. Ferrari. 2005. Forrajeras y pasturas del ecosistema templado húmedo de la Argentina. 2ª Ed. UN de Lomas de Zamora. Argentina. 522 pp.

Milano, V.A. 1977. Identificación de las especies forrajeras de Lotus por sus semillas. IDIA 355. pp 58-59.

Montes, L. 1992. Morphological and agronomical characterization of Lotus corniculatus. Lotus Newsletter pp 6-8.

Pallares, L., L. Ferrari, & M. Ritta. 2000. Discrimination between seed storage proteins of Lotus tenuis and Lotus corniculatus by P.A.G.E. Seed Science and Technology 28(3). pp 769-777.

Peretti, A. 1994. Manual para análisis de semillas. Hemisferio Sur. Buenos Aires. Argentina. 282 pp.

Steel, R.G.D. & J.H. Torrie. 1988. Bioestadística. Principios y procedimientos. 1ª ed. español. Mc Graw Hill. México. 622 pp.

Vignolio, O.R., N.O. Maceira & O.N. Fernandez. 1996. Efecto del anegamiento estacional sobre la reproducción de Lotus tenuis y Lotus corniculatus. Revista Argentina de Producción Animal. Asociación Argentina de Producción Animal. 16 (1) pp 250-251.

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Published

2015-06-15

How to Cite

Murcia, M. L., Peretti, A., & San Martino, S. (2015). Morphological characterization of seeds and seedlings of Lotus tenuis and Lotus corniculatus in order to differentiate species in seed samples. Journal of the Agronomy College, 114(1), 38-43. https://revistas.unlp.edu.ar/revagro/article/view/20177

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