Porcine Escherichia coli isolates resistant to critically important antimicrobials for public health
DOI:
https://doi.org/10.24215/15142590e067Keywords:
critically important antimicrobials, Escherichia coli, pigs, antimicrobial resistanceAbstract
The goal of this study was to establish the behavior of cefotaxime (CTX)-resistant and/or colistin-resistant E. coli, isolated from fecal samples of pigs in Argentina to other antimicrobials classified by the WHO as critically important for public health. In 2018, 56 samples from 11 farms located in Buenos Aires, Córdoba, Santa Fe and San Luis provinces were processed. Thirty-one cefotaxime-resistant E. coli, 21 colistin-resistant E. coli, and 23 cefotaxime-colistin-resistant E. coli were obtained. Sixty-nine percent of the E. coli isolated were resistant to nalidixic acid and 37.3 % to ciprofloxacin, antimicrobials classified as the highest priority. No carbapenem-resistant E. coli were isolated. Seventy-three isolates were classified as multiresistant, of which 50 % showed resistance against 6 or 7 antimicrobial groups. Third-generation cephalosporin resistance was mainly associated with the CTX-M gene, particularly CTX-M-8/25, while colistin resistance was associated with the mcr-1 gene. The study evidences that Argentinian pigs harbor multidrug-resistant E. coli isolates, including resistance toantimirobials of critical importance for human and veterinary medicine. Complementary analyses will be necessary to determine extended-spectrum β-lactamase-producing E. coli clones circulating in pig farms and their implications for public health.
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Benavides JA, Salgado-Caxito M, Opazo-Capurro A, González Muñoz P, Piñeiro A, Otto Medina M, Rivas L, Munita J, Millán J. 2021. ESBL-producing Escherichia coli carrying CTX-M genes circulating among livestock, gogs, and wild mammals in small-scale farms of central Chile. Antibiotics. 10(5): 510 https://doi.org/10.3390/antibiotics10050510
Benklaouz BM, Aggad H, Benameur Q. 2020. Resistance to multiple first-line antibiotics among Escherichia coli from poultry in Western Algeria. Veterinary World. 13(2):290-5. https://doi.org/10.14202/vetworld.2020.290-295
Bergšpica I, Kaprou G, Alexa EA, Prieto M, Alvarez-Ordóñez A. 2020. Extended spectrum-lactamase (ESBL) producing Escherichia coli in pigs and pork meat in the European Union. Antibiotics 9(10): 678 https://doi.org/10.3390/antibiotics9100678
Clinical and Laboratory Standards Institute. CLSI M100-ED31:2021 Performance Standards for Antimicrobial Susceptibility Testing, 31st Edition.
Faccone D, Moredo FA, Giacoboni GI, Albornoz E, Alarcón LV, Nievas VF, Corso A. 2019. Multidrug-resistant Escherichia coli harbouring mcr-1 and blaCTX-M genes isolated from swine in Argentina. Journal of Global Antimicrobial Resistance. 18:160-2. https://doi.org/10.1016/j.jgar.2019.03.011
Faccone D, Rapoport M, Albornoz E, Celaya F, De Mendieta J, De Belder D, Lucero C, Gómez S, Danze D, Pasteran F, Corso A. 2020. Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018. Revista Panamericana de Salud Pública. 44:e55. https://doi.org/10.26633/RPSP.2020.55
Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang H, Liu JH, Shen J. 2016. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. The Lancet Infectious Diseases. 16(2):161-8. https://doi.org/10.1016/s1473-3099(15)00424-7
Madec JY, Haenni M, Nordmann P, Poirel L. 2017. Extended-spectrum β-lactamase/AmpC- and carbapenemase producing Enterobacteriaceae in animals: a threat for humans? Clinical Microbiology and Infection 23(11):826-33. https://doi.org/10.1016/j.cmi.2017.01.013
Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL, Rice LB, Stelling J, Struelens MJ, Vatopoulos A, Weber JT, Monnet DL. 2012. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection 18(3):268-81. https://doi.org/10.1111/j.1469-0691.2011.03570
Moredo FA, Piñeyro PE, Márquez GC, Sanz M, Colello R, Etcheverría A, Padola NL, Quiroga MA, Perfumo CJ, Galli L, Leotta GA. 2015. Enterotoxigenic Escherichia coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles. Foodborne Pathogens and Diseases. 12(8):704-11. https://doi.org/10.1089/fpd.2015.1959
Organización Mundial de la Salud. 2017. Directrices de la OMS sobre el uso de antimicrobianos de importancia médica en animales destinados a la producción de alimentos. Disponible en: http://antimicrobianos.com.ar/ATB/wp-content/uploads/2017/11/WHO-NMH-FOS-FZD-17.4-spa.pdf [Consultado 30/07/2021].
Organización Mundial de la Salud. 2018. 6.a Revisión de la Lista OMS de antimicrobianos de importancia crítica para la medicina humana. Disponible en: https://apps.who.int/iris/bitstream/handle/10665/325037/WHO-NMH-FOS-FZD-19.1-spa.pdf?ua=1 [Consultado 30/07/2021].
Organización Mundial de Sanidad Animal. 2021. Lista de agentes antimicrobianos importantes para la medicina veterinaria. Disponible en: https://www.oie.int/app/uploads/2021/06/e-oie-lista-antimicrobianos-junio2021.pdf
Pagani L, Dell’Amico E, Migliavacca R, D’Andrea M, Giacobone E, Amicosante G, Romero E, Rossolini G. 2003. Multiple CTX-M-Type extended-spectrum β-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in Northern Italy. Journal Clinical Microbiology. 41(9):4264-9. https://doi.org/10.1128/JCM.41.9.4264-4269.2003
Pissetti C, Kich JD, Allen HK, Navarrete C, de Freitas Costa E, Morés N, Cardoso M. 2021. Antimicrobial resistance in commensal Escherichia coli and Enterococcus spp. isolated from pigs subjected to different antimicrobial administration protocols. Research in Veterinay Science. 137:174-85. https://doi.org/10.1016/j.rvsc.2021.05.001
Ramos S, Silva V, Dapkevicius MLE, Caniça M, Tejedor-Junco MT, Igrejas G, Poeta P. 2020. Escherichia coli as commensal and pathogenic bacteria among food-producing animals: health implications of extended spectrum β-lactamase (ESBL) production. Animals. 10(12):2239. https://doi.org/10.3390/ani10122239
Servicio de Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas INEI, ANLIS “Carlos Malbrán”. 2017. Método de screening “COLISTIN AGAR-SPOT”. http://antimicrobianos.com.ar/ATB/wp-content/uploads/2017/09/Protocolo-Agar-spot-COL-2017-version2-Agosto2017.pdf
Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Resolución SENASA 591/2015. Disponible en: http://www.senasa.gob.ar/sites/default/files/r_senasa_591-2015.pdf
Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Resolución 22/2019. Disponible en: https://www.boletinoficial.gob.ar/detalleAviso/primera/200151/20190115
Shafiq M, Huang J, Rahman SU, Shah JM, Chen L, Gao Y, Wang M, Wang L. 2019. High incidence of multidrug-resistant Escherichia coli coharboring mcr-1 and blaCTX-M-15 recovered from pigs. Infection and Drug Resistance. 12:2135-49. https://doi.org/10.2147/IDR.S209473
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Copyright (c) 2022 María Fernanda Gómez, Florencia Vinocur, Sofía Rodríguez Ramos, Bárbara Julieta Garassino, Hernán Darío Nievas, Victorio Fabio Nievas, Laura Valeria Alarcón, Alberto Domingo Armocida, Estefanía Marisol Pérez, David Griffo, Gabriela Isabel Giacoboni, Fabiana Alicia Moredo
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