Molecular characterization of infectious bronchitis virus in laying hen farms located in Tungurahua province, Ecuador
DOI:
https://doi.org/10.24215/15142590e066Keywords:
Avian infectious bronchitis virus, laying hens, isolates, phylogeny, vaccinesAbstract
Avian infectious bronchitis (IBV) generates significant economic losses. While vaccination decreases these losses, new viral strains are continually emerging. This research aimed to perform molecular characterization of IBV strains circulating in laying hen farms in Tungurahua province, Ecuador. Swabs and organ samples from 47 farms were collected and sequenced, and results were compared to 17 live vaccines used in the area Sixteen farms were positive for IBV as determined by amplification of the 5'UTR region and a S1 segment. The construction of a phylogenetic tree showed that 5 samples were in the same clade as the Massachusetts-type strains, linage GI-1; 7 samples were in the GI-13 lineage clade of vaccine type 793B strains, and 4 samples were into a clade of Q1 type strains. In conclusion, although studies that include the entire protein S sequence are necessary, we were able to detect three types of strains circulating in the province of Tungurahua, two of which are probably vaccine-derived and one has an unknown origin.
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