Liquid culture medium for diagnosis of bovine paratuberculosis. Usefulness and comparative analysis with Herrold medium: preliminary results

Authors

  • Magali Andrea Romero Centro de Diagnóstico e Investigaciones Veterinarias
  • María Fiorella Alvarado Pinedo Centro de Diagnóstico e Investigaciones Veterinarias
  • Luis Maria Peralta Centro de Diagnóstico e Investigaciones Veterinarias
  • Pedro Sebastián Sosa Centro de Diagnóstico e Investigaciones Veterinarias
  • Roberto Damián Moyano Instituto Nacional de Tecnología agropecuaria (INTA), laboratorio de biotecnología. Castelar.
  • Maria de la Paz Santangelo Instituto Nacional de Tecnología agropecuaria (INTA), laboratorio de biotecnología. Castelar.
  • Gabriel Eduardo Travería Centro de Diagnóstico e Investigaciones Veterinarias

DOI:

https://doi.org/10.24215/15142590e025

Keywords:

Mycobacterium, liquid culture medium, Ziehl-Neelsen, mycobactin

Abstract

Paratuberculosis is a chronical disease caused by acidfast bacilli (AFB) Mycobacterium avium subsp. paratuberculosis (Map). There is neither an effective treatment nor any vaccine approved by the sanitary authority (SENASA) in Argentina. Diagnosis and segregation of positive animals are essential for the control of the disease. For the diagnosis of cattle paratuberculosis, a modified Middlebrook M7H9 broth medium was developed. To test the effectiveness of the medium, fecal and tissue samples were cultured in Herrold with mycobactin and liquid culture media, and time to growth was compared by direct observation of colonies and microscopic grouped AFB observation, respectively. Significant differences were observed between liquid tested and Herrold media, both in time to grow and in the number of positive animals detected. Based on these results, it is concluded that liquid medium offers greater analytical sensitivity and faster detection than Herrold medium.

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Published

2018-06-22

How to Cite

Romero, M. A., Alvarado Pinedo, M. F., Peralta, L. M., Sosa, P. S., Moyano, R. D., Santangelo, M. de la P., & Travería, G. E. (2018). Liquid culture medium for diagnosis of bovine paratuberculosis. Usefulness and comparative analysis with Herrold medium: preliminary results. Analecta Veterinary, 38(1), 50–55. https://doi.org/10.24215/15142590e025

Issue

Vol. 38 No. 1 (2018): Analecta Veterinaria

Section

Technical reports

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Analecta Veterinaria by School of Veterinary Sciences, National University of La Plata is distributed under a Creative Commons Attribution-NonCommercial-NoDeriv 4.0 International License.

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