Producción de la glicoproteína G del virus de la rabia en la levadura Pichia pastoris, para su uso en medicina veterinaria

Leandro Daniel Picotto; Guillermo Hernán Sguazza; Marco Antonio::http://voc.sedici.unlp.e Tizzano; Sebastián Fernando::http://voc.sedici.u Cavalitto; Marcelo Ricardo Ítalo Pecoraro

doi:10.24215/15142590ep. 5-11

Producción de la glicoproteína G del virus de la rabia en la levadura Pichia pastoris, para su uso en medicina veterinaria

Authors

Leandro Daniel Picotto
Guillermo Hernán Sguazza
Marco Antonio::http://voc.sedici.unlp.e Tizzano
Sebastián Fernando::http://voc.sedici.u Cavalitto
Marcelo Ricardo Ítalo Pecoraro

DOI:

https://doi.org/10.24215/15142590ep.%205-11

Keywords:

glicoproteína

Abstract

The Glycoprotein (G) gene of rabies virus CVS-51 strain was cloned, sequenced and expressed in Pichia pastoris yeast as a secreted protein. The antigenicity of this recombinant protein was preliminarily examined by Western blot. Like native glycoprotein, the recombinant glycoprotein was found reactive when challenged against specific antibodies. The data indicate that the recombinant protein can be expressed and isolated straightforwardly and may be useful as a safe source in the production of diagnostic kits and subunit vaccines to prevent the disease.

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Published

2015-12-30

How to Cite

Picotto, L. D., Sguazza, G. H., Tizzano, . M. A., Cavalitto, . S. F., & Pecoraro, M. R. Ítalo. (2015). Producción de la glicoproteína G del virus de la rabia en la levadura Pichia pastoris, para su uso en medicina veterinaria. Analecta Veterinary, 35(2), p. 5–11. https://doi.org/10.24215/15142590ep. 5-11

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Issue

Vol. 35 No. 2 (2015)

Section

Research Articles

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Analecta Veterinaria by School of Veterinary Sciences, National University of La Plata is distributed under a Creative Commons Attribution-NonCommercial-NoDeriv 4.0 International License.