Prevalencia del virus diminuto del ratón, determinada mediante una técnica de PCR semianidada, en ratones de experimentación de bioterios de Argentina

Virus diminuto del ratón en bioterios de Argentina

  • Juan Martín Laborde Docente, FCV,UNLP
  • Guillermo Hernan Sguazza Laboratorio de Virología (LAVIR); Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • Nadia Analia Fuentealba Laboratorio de Virología (LAVIR); Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-CCT-La Plata)
  • Santiago Gerardo Corva Cátedra de Bioestadística, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • Cecilia Monica Galosi Laboratorio de Virología (LAVIR); Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Argentina.
  • Cecilia Carbone Cátedra de Animales de Laboratorio, Laboratorio de animales de experimentación (LAE), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
Palabras clave: virus diminuto del ratón, PCR, prevalencia

Resumen

El virus diminuto del ratón puede producir alteraciones de los parámetros fisiológicos de los animales infectados, lo que hace que se modifiquen los resultados de aquellas experiencias en las que el ratón es utilizado como modelo experimental. Para poner en práctica medidas adecuadas de control del virus en bioterios, son necesarios métodos de detección eficientes, ya sea técnicas serológicas que ponen en evidencia la presencia de anticuerpos antivirales en el huésped, o técnicas moleculares como la PCR. El objetivo de este trabajo fue diseñar y poner a punto una técnica de PCR semianidada para la detección de ADN viral a partir de muestras de materia fecal y de bazo y, determinar, mediante esta técnica molecular, la prevalencia del virus en bioterios de Argentina. Diecinueve muestras de pools de heces (del 41,3 % de los bioterios) y 109 muestras de bazo (del 47,82 % de los bioterios) resultaron positivas. La prevalencia a partir del análisis total de las muestras de bazo fue estimada en 23,69 %. La técnica de PCR semianidada diseñada, constituye una herramienta molecular sensible y específica para realizar la detección del virus diminuto del ratón en instalaciones de animales de experimentación de Argentina.

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Publicado
2020-06-30
Cómo citar
Laborde, J. M., Sguazza, G. H., Fuentealba, N. A., Corva, S. G., Galosi, C. M., & Carbone, C. (2020). Prevalencia del virus diminuto del ratón, determinada mediante una técnica de PCR semianidada, en ratones de experimentación de bioterios de Argentina . Analecta Veterinaria, 40(1), 046. https://doi.org/10.24215/15142590e046
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Trabajos de Investigación