Detection of antibodies against Brucella abortus in dogs in contact with rural areas
Zoonotic aspects of the infection
DOI:
https://doi.org/10.24215/15142590e038Keywords:
Brucella abortus, dogs, zoonoses, ArgentinaAbstract
Brucellosis is a zoonotic-contagious disease, usually of chronic evolution and worldwide distribution.
Dog’s infection is typically caused for B. canis; however, B. abortus, associated with infected cattle has
been described in dogs, both experimentally and in field conditions. The transmission of dogs to humans
is also possible. This communication describes, for the first time in Argentina, the detection of antibodies
against B. abortus in a population of dogs in contact with rural areas. Sixtyseven samples of dog sera
were analyzed, of which 26.8 % were positive for B. abortus by means of the agglutination technique with
buffered antigen, 7.5 % were confirmed as positive by tests of slow agglutination in tubes and 4, 5 % were
positive by polarized fluorescence test. Dogs reacting to B. abortus had contact with seropositive cattle
with brucellosis or were urban dogs that lived with dogs that had attended the rural area. Elimination of
infected cattle may not necessarily eradicate brucellosis; management actions such as avoiding the
contact of dogs from infected cows should prevent its transmission.
References
Acha PN, Szyfres B. 2001. Zoonosis y enfermedades transmisibles comunes al hombre y a los animales. Brucellosis. 3° Ed. Washington: OPS/OMS, pp. 28-56 (Publicación Científica y Técnica, 580).
Alton GG, Jones LM, Angus RD, Verger JM. 1988. Techniques for the brucellosis Laboratory. Institute National de la Recherche Agronomique (INRA). Paris, p. 190.
Araoz L, Cruz ML, González del Pino F, Jorrat JJ, de la Vega AC. 2014. Estudio comparativo de distintas técnicas serológicas para el diagnóstico de brucelosis equina. Revista Agronómica del Noroeste Argentino. 34(2):177-9.
Azevedo SS, Batista CSA, Alves CJ, Clementino LJ. 2003. Ocorrência de anticorpos contra Brucella abortus em cães errantes da cidade de Patos, Estado da Paraíba, Brasil. Arquivos do Instituto Biológico, São Paulo. 70(4):499-500.
Baek BK, Lim CW, Rahman MS, Kim CH, Oluoch A, Kakoma I. 2003. Brucella abortus infection in indigenous Korean dogs. Canadian Journal of Veterinary Research. 67(4):312-4.
Barr SC, Eilts BE, Roy AF, Miller R. 1986. Brucella suis biotype 1 infection in a dog. Journal of the American Veterinary Medical Association. 189(1):686-7.
Bicknell SR, Bell RA, Richards PA. 1976. Brucella abortus in the bitch. Veterinary Record. 99(5):85-6. doi: 10.1136/vr.99.5.85
Carmichael LE, Zoha SJ, FloresCastro R. 1984a. Biological properties and dog response to a variant (M) strain of Brucella canis. Developments in Biological Standarization. 56:649-56.
Carmichael LE, Zoha SJ, FloresCastro R. 1984b. Problems in the serodiagnosis of canine brucellosis: dog responses to cell wall and internal antigens of Brucella canis. Developments in Biological Standarization. 56:371-83.
Carmichael LE, Joubert JC. 1987. A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant (M) organism as antigen. Cornell Veterinarian. 77(1):3-12.
Carmichael LE, Greene CE. 1998. Canine Brucellosis. En: Greene, C.E. (Ed). Infectious diseases of the dog and cat. 2° Ed. Philadelphia, W.B. Saunders, pp. 248-57.
Cutler SJ, Fooks AR, van der Poel WHM. 2010. Public health threat of new, reemerging, and neglected zoonoses in the industrialized world. Emerging Infectious Diseases. 16(1):1-7. doi: 10.3201/eid1601.081467
Day M. 2011. One health: the importance of companion animal vectorborne diseases. Parasites & Vector. 4:49-55. doi: 10.1186/17563305449
Eiras DF, Scodellaro C, Vezzani D, López G, Boero C, Sánchez R. 2014. Diagnóstico serológico de brucelosis en perros del conurbano sur bonaerense. Revista Argentina de Zoonosis y Enfermedades Infecciosas Emergentes 9(2):26-7.
Forbes LB. 1990. Brucella abortus infection in 14 farm dogs. Journal of the American Veterinary Medical Association. 196(6):911-6.
Gall D, Nielsen K, Bermúdez RM, Muñóz Del Real MC, Halbert G, Groulx R, Moreno F, Chow EY, Checkley SL. 2006. Development of an indirect enzymelinked immunosorbent assay for detecting equine serum antibodies to the lipopolysaccharide of Salmonella abortus equi. Research in Veterinary Sciences. 81(2):215-7. doi: 10.1016/j.rvsc.2005.11.004
Godfroid J, Saegerman C, Wellemans V. 2002. How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Veterinary Microbiology. 90(14):461-77. doi: 10.1016/S03781135(02)002304
Godfroid J, Al Dahouk S, Pappas G, Roth F, Matope G, Muma J, Marcotty T, Pfeiffer D, Skjerve E. 2013. A “One Health” surveillance and control of brucellosis in developing countries: moving away from improvisation. Comparative Immunology, Microbiology and Infectious Diseases. 36(3): 241-8. doi: 10.1016/j.cimid.2012.09.001
Hensel ME, Negron M, Arenas Gamboa, AM. 2018. Brucellosis in dogs and public health risk. Emerging Infectious Diseases. 24(8):1401-6. doi: 10.3201/eid2408.171171
Johnson CA, Bull RW, Schirmer RG. 1983. Peripheral lymphocyte function in dogs with Brucella canis infection. Veterinary Immunology and Immunopathology. 4(4):425-31.
Kose S, Serin Senger S, Akkoclu G, Kuzucu L, Ulu Y, Ersan G, Ouz F. 2014. Clinical manifestations, complications, and treatment of brucellosis: evaluation of 72 cases. Turkish Journal of Medical Sciences. 44(2):220-3. doi: 10.3906/sag111234
Krueger WS, Lucero NE, Brower A, Heil GL, Gray GC. 2014. Evidence for unapparent Brucella canis infections among adults with occupational exposure to dogs. Zoonoses Public Health 61(7):509-18. doi: 10.1111/zph.12102
MainarJaime RC, Muñóz PM, de Miguel MJ, Grilló MJ, Marín CM, Moriyón I, Blasco JM. 2005. Specificity dependence between serological tests or diagnosing bovine brucellosis in Brucella free farms showing false positive serological reactions due to Yersinia enterocolítica O:9. The Canadian Veterinary Journal. 46(10):913-6.
MateudeAntonio EM, Martin M, Casal J. 1994. Comparison of serologic tests used in canine brucellosis diagnosis. Journal of Veterinary Diagnostic Investigation. 6(2):257-9. doi: 10.1177/104063879400600220
Maudlin I, Eisler MC, Welburn SC. 2009. Neglected and endemic zoonoses. Philosophical transactions of the Royal Society of London. Series B, Biological Sciences. 364(1530):2777-87. doi: 10.1098/rstb.2009.0067
McDermott J, Grace D, Zinsstag J. 2013. Economics of brucellosis impact and control in lowincome countries. Revue Scientifique et Technique. 32(1):249-61.
McGiven JA, Tucker JD, Perrett LL, Stack JA, Brew SD, MacMillan AP. 2003. Validation of FPA and cELISA for the detection of antibodies to Brucella abortus in cattle sera and comparison to SAT, CFT, and iELISA. Journal of Immunological
Methods. 278(12):171-8. doi: 10.1016/S00221759(03)002011
Miranda KL, Cottorello ACP, Poester FP, Lage AP. 2005. Brucelose canina. En: Cadernos Técnicos de Veterinária e Zootecnia. Conselho Regional de Medicina Veterinária de Minas Gerais, Brasil (Editores). n.47, pp. 66-82.
Moreno E. 2014. Retrospective and prospective perspectives on zoonotic brucellosis. Frontiers in Microbiology. 13(5):213. doi: 10.3389/fmicb.2014.00213
Nicola A, Elena S. 2009. Manual de diagnóstico serológico de la brucelosis bovina. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Buenos Aires, Argentina. p. 95.
Nielsen K. 2002. Diagnosis of brucellosis by serology. Veterinary Microbiology. 90(14):447-59. doi: 10.1016/S03781135(02)002298
Nielsen K, Smith P, Yu W, Nicoletti P, Elzer P, Robles C, Bermúdez R, Renteria T, Moreno FS, Ruiz A, Massengill C, Muenks Q, Jurgersen G, Tollersrud T, Samartino L, Conde S, Forbes L, Gall D, Perez B, Rojas X, Minas A. 2005. Towards
single screening tests for brucellosis. Revue Scientifique et Technique. 24(3):1027-37.
Olsen SC, Palmer MV. 2014. Advancement of knowledge of Brucella over the past 50 years. Veterinary Pathology 51(6):1076–89. doi: 10.1177/0300985814540545
Pidgeon GL, Scanlan CM, Miller WR, Mayer TW. 1987. Experimental infection of dogs with Brucella
abortus. The Cornell Veterinarian. 77(4):339-47.
Prior MG. 1976. Isolation of Brucella abortus from two dogs in contact with bovine brucelosis. Canadian Journal of Comparative Medicine. 40(1):117-8.
Ramamoorthy S, Woldemeskel M, Ligett A, Snider R, Cobb R, Rajeev S. 2011. Brucella suis infection in dogs, Georgia, USA. Emerging Infectious Diseases. 17(12):2386-87. doi: 10.3201/eid1712.111127
Robles, CA.1998. Evaluación de una técnica de doble difusión en gel de agar para el diagnóstico de la infección por Brucella ovis en carneros. Veterinaria Argentina. Vol XV(142):119-24.
Sandoval LA, Conrado Ribeiro LO, Amaral LBS, Feitosa MH, Bazan JM. 1976. Incidência da Brucelose canina na Cidade de São Paulo. Biológico, v.42, pp.128-32.
Shin SJ, Carmichael L. 1999. Canine brucellosis caused by Brucella canis. En: Carmichael L. Ed. Recent advances in canine infectious diseases. Ithaca, IVIS (www.ivis.org) A0101.1199.
Uçan US, Aras Z. 2015. The “One Health” concept and a need for national monitoring of canine and feline infections. En: Proceedings of the 1st Turkish Congress of One Health. Konya, Turkey, pp. 61-2.
Vargas F. 2002. Brucellosis in Venezuela. Veterinary Microbiology. 90(14):39-44. doi: 10.1016/S03781135(02)002432
Whatmore AM. 2009. Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infection, Genetics and Evolution. 9(6):1168-84. doi: 10.1016/j.meegid.2009.07.001
Whatmore AM, Davison N, Cloeckaert A, Al Dahouk S, Zygmunt MS, Brew SD, Perrett LL, Koylass MS, Vergnaud G, Quance C, Scholz HC, Dick EJ Jr, Hubbard G, Schlabritz-Loutsevitch NE. 2014. Brucella papionis sp. nov., isolated from baboons (Papio spp.). International Journal of Systematic and Evolutionary Microbiology. 64(12):4120-8. doi: 10.1099/ijs.0.0654820
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