Effect of folic acid and carboplatin treatment on the viability of non-tumoral cells
DOI:
https://doi.org/10.24215/18536387e094Keywords:
micronutrients, chemotherapeutic agents, cell viabilityAbstract
Cancer cells are highly adaptive and often resistant to antitumor agents, which prevents effective cancer therapy. However, in these cells there are factors that can modify the response to chemotherapy treatment. Previous results, obtained in HeLa tumor cells, demonstrated that folic acid (FA) combined with carboplatin (CBP) allowed lowering the dose of CBP used and increasing the activity of the platinum compound. In this sense, it is essential to evaluate the effects of such treatment on healthy cells. For this reason, the effect of combined FA and CBP treatment in vitro on cell viability (MTT assay) in peripheral blood of healthy women was analyzed. Cultures were carried out for 48 hours at 37°C, during the last 24 hours the treatments were carried out: 1. negative control (NC); 2. FA control (900 nM); 3. CBP control (40.4 mM); 4. mannitol control (ML 40.4 mM); 5. combined FA-CBP (900 nM-40.4 mM); 6. combined FA-ML (900 nM-40.4 mM); 7. positive control (PC ethanol 10%). The cultures that received the FA-CBP combination showed a similar viability to that observed for NC. On the contrary, in the cultures that received treatment with CBP only, viability decreased in a statistically significant manner compared to the control. These findings could be a contribution to exploring the use of FA in protocols based on platinum agents, in order to reduce doses in the treatment of patients and the appearance of side effects.
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