Arqueoproteómica como complemento de estudios paleopatológicos en restos óseos humanos de la Cueva de Plaza, Chubut, Argentina: alcances y limitaciones

Ricardo Martín Neme Tauil; Denise Evans; Paula Miranda De Zela; Silvia Moreno; Fabián Crespo; Vivian Scheinsohn

doi:10.24215/18536387e062

Authors

Ricardo Martín Neme Tauil Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Universidad de Buenos Aires (UBA). https://orcid.org/0000-0001-8018-6068
Denise Evans Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación. Instituto Nacional de Antropología y Pensamiento Latinoamericano (INAPL). https://orcid.org/0000-0002-9936-0780
Paula Miranda De Zela Banco Nacional de Datos Genéticos (BNDG). https://orcid.org/0000-0003-1335-3473
Silvia Moreno Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Universidad de Buenos Aires (UBA). https://orcid.org/0000-0003-3876-2951
Fabián Crespo Department of Anthropology, Center of Archaeology and Cultural Heritage, University of Louisville, KY, EEUU https://orcid.org/0000-0002-4923-7338
Vivian Scheinsohn Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Universidad de Buenos Aires (UBA). https://orcid.org/0000-0002-6097-331X

DOI:

https://doi.org/10.24215/18536387e062

Keywords:

proteomics, bioarchaeology, paleopathology, Patagonia

Abstract

In this work a proteomic analysis by mass spectrometry of a human phalanx with a macroscopic alteration found in Cueva de Plaza archaeological site (Chubut, Argentina) is proposed, representing the first time that such analysis has been carried out on archaeological materials in Argentina. Two methods of protein extraction from archaeological bone remains are presented and their results were analyzed with a mass spectrometer. Both methods yielded non-exclusive, partially redundant and complementary results. Collagens, fibronectin, proteins from cartilage, bone, muscle and blood and, to a lesser extent, proteins associated with the immune system and other metabolic pathways were identified. These proteins are consistent with the existence of a healing trauma process in the analyzed bone element.

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References

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