Arqueoproteómica como complemento de estudios paleopatológicos en restos óseos humanos de la Cueva de Plaza, Chubut, Argentina: alcances y limitaciones

Ricardo Martín Neme Tauil; Denise Evans; Paula Miranda De Zela; Silvia Moreno; Fabián Crespo; Vivian Scheinsohn

doi:10.24215/18536387e062

Autores/as

Ricardo Martín Neme Tauil Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Universidad de Buenos Aires (UBA). https://orcid.org/0000-0001-8018-6068
Denise Evans Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación. Instituto Nacional de Antropología y Pensamiento Latinoamericano (INAPL). https://orcid.org/0000-0002-9936-0780
Paula Miranda De Zela Banco Nacional de Datos Genéticos (BNDG). https://orcid.org/0000-0003-1335-3473
Silvia Moreno Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Universidad de Buenos Aires (UBA). https://orcid.org/0000-0003-3876-2951
Fabián Crespo Department of Anthropology, Center of Archaeology and Cultural Heritage, University of Louisville, KY, EEUU https://orcid.org/0000-0002-4923-7338
Vivian Scheinsohn Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Universidad de Buenos Aires (UBA). https://orcid.org/0000-0002-6097-331X

DOI:

https://doi.org/10.24215/18536387e062

Palabras clave:

proteómica, bioarqueología, paleopatología, Pampa-Patagonia

Resumen

En este trabajo se propone realizar por primera vez en Argentina un análisis proteómico por espectrometría de masas de una falange humana con una alteración macroscópica proveniente de un conjunto arqueológico recuperado en el sitio Cueva de Plaza (Chubut, Argentina). Se presentan dos métodos de extracción de proteínas de restos óseos arqueológicos para ser analizadas con un espectrómetro de masas. Ambos métodos permitieron obtener resultados no excluyentes con información parcialmente redundante y complementaria. Se identificaron proteínas como colágenos, fibronectina, proteínas de cartílago, de hueso, de músculo y de sangre y conjuntos de proteínas asociadas al sistema inmune y a otras vías metabólicas, en menor cantidad. Las proteínas identificadas son consistentes con la existencia de un trauma en proceso de reparación en el elemento óseo analizado.

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