Citomolecular effect of vitamin E and ferrous sulfate: an in vitro model on preventive treatme
DOI:
https://doi.org/10.24215/18536387e031Keywords:
ferrous sulfate, vitamin E, micronucleousAbstract
Iron deficiency is one of the most prevalent deficiencies during childhood, and also one of the main causes of anemia. Since anemia affects normal child growth and development, the Argentine Society of Pediatrics recommends preventive daily supplementation with ferrous sulfate. However, daily ferrous sulfate treatment may induce iron tissue overload, resulting in damage to proteins, lipids, and DNA. In order to avoid these undesired effects, a possible alternative is to implement weekly ferrous sulfate supplementation. Vitamin E is the main liposoluble non-enzymatic antioxidant for cell defense. This study evaluated the combined effect of vitamin E and ferrous sulfate supplementation (daily and weekly) on human peripheral blood lymphocytes cultured in vitro using the cytokinesis-blocked micronucleus assay. Eight treatments were conducted, including controls and combined treatments of daily and weekly ferrous sulfate with vitamin E. Differences were significant for micronucleus frequency (F= 840.04; p= 0.0). In conclusion, a protective effect of vit E on cell components with lower genotoxic damage on weekly ferrous sulfate treatment could be observed.
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