Efecto de la adición de dimetilformamida al diluyente tris base sobre la supervivencia de espermatozoides felinos congelados-descongelados
DOI:
https://doi.org/10.24215/15142590ep.%2014-19Abstract
The aim of this study was to assess the effect of dimethylformamide in replacement of a glycerol part in a Tris-based extender on viability of frozen-thawed feline sperm. Twenty male cats were included in this study. Sixteen cats were castrated and epididymides were used for spermatozoa recovery. The remaining 4 animals were used for obtained semen by electroejaculation. Cryopreservation with sperm obtained from epididymides and semen were done using a Tris-based extender with 0.5% or 0% of dimethylformamide. The addition of dimethylformamide does not improve sperm parameters compared whit sperm frozen with Tris based extender. The addition of a higher percentage of this amide could show the protector effect on frozen thawed feline sperm.
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