Prevalence of infections produced by the minute virus of mice, determined by hemi-nested PCR, in mice of conventional facilities of Argentina
Virus diminuto del ratón en bioterios de Argentina
DOI:
https://doi.org/10.24215/15142590e046Keywords:
Minute virus of mice, PCR, prevalenceAbstract
The minute virus of mice may alter physiological parameters of infected animals, which can lead to modifications in research results when mice are used as an experimental model. Efficient detection methods are necessary to implement adequate virus control measures in mice facilities, either by serological techniques that reveal the presence of antiviral antibodies in the host, or by molecular techniques such as PCR. The purpose of this study was to design and standardize a heminested PCR technique to detect viral DNA from stool and spleen samples and to determine the prevalence of the virus in conventional mice facilities of Argentina by this molecular technique. Nineteen pools of stool (from 41.3 % of conventional facilities) and 109 spleens (from 47.82 % of conventional facilities) were positive. The prevalence from the total analysis of the spleen samples was estimated at 23.69 %. The designed heminested PCR was sensitive and specific and allows the detection of minute virus of mice in experimental mice facilities of Argentina.
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